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Product Info Summary
|Description:||Transient overexpression lysate of valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1)|
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VCIP 135 (VCPIP1) (NM_025054) Human Over-expression Lysate
Transient overexpression lysate of valosin containing protein (p97)/p47 complex interacting protein 1 (VCPIP1)
Storage & Handling
Cite This Product
VCIP 135 (VCPIP1) (NM_025054) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080124)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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VCIP 135 (VCPIP1) Human Over-expression Lysates NM_025054.
Western blot validation of overexpression lysate (LS080124) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with VCPIP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for VCPIP1 (Source: Uniprot.org, NCBI)
Deubiquitinating protein VCIP135
DKFZp686G038; DUBA3; EC 3.4.22.-; FLJ23132; KIAA1850FLJ60694; valosin containing protein (p97)/p47 complex interacting protein 1; valosin-containing protein (p97)/p47 complex-interacting protein p135; Valosin-containing protein p97/p47 complex-interacting protein 1; Valosin-containing protein p97/p47 complex-interacting protein p135; VCIP135; VCIP135deubiquitinating protein VCIP135; VCPIP1 VCIP135; VCPIP1 VCPIP1 DUBA3, VCIP135 valosin containing protein interacting protein 1 deubiquitinating protein VCPIP1|VCP/p47 complex-interacting 135-kDa protein|deubiquitinating protein VCIP135|valosin-containing protein p97/p47 complex-interacting protein 1|valosin-containing protein p97/p47 complex-interacting protein p135*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on VCPIP1, check out the VCPIP1 Infographic
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No publications found for LS080124
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