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Product Info Summary
|Description:||Transient overexpression lysate of solute carrier family 18 (vesicular monoamine), member 2 (SLC18A2)|
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VMAT2 (SLC18A2) (NM_003054) Human Over-expression Lysate
Transient overexpression lysate of solute carrier family 18 (vesicular monoamine), member 2 (SLC18A2)
Storage & Handling
Cite This Product
VMAT2 (SLC18A2) (NM_003054) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006571)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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VMAT2 (SLC18A2) Human Over-expression Lysates NM_003054.
Western blot validation of overexpression lysate (LS006571) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC18A2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC18A2 (Source: Uniprot.org, NCBI)
Synaptic vesicular amine transporter
major facilitator superfamily
MGC120477; MNAT; monoamine neurotransmitter transporter; Monoamine transporter; SLC18A2; solute carrier family 18 (vesicular monoamine), member 2; Solute carrier family 18 member 2; SVAT; SVMT; SVMTMGC120478; synaptic vesicular amine transporter; VAT2; VAT2MGC26538; vesicle monoamine transporter type 2; vesicle monoamine/H+ antiporter; Vesicular amine transporter 2; VMAT2 SLC18A2 PKDYS2, SVAT, SVMT, VAT2, VMAT2 solute carrier family 18 member A2 synaptic vesicular amine transporter|monoamine neurotransmitter transporter|solute carrier family 18 (vesicular monoamine transporter), member 2|synaptic vesicle amine transporter, brain|synaptic vesicle monoamine transporter, brain|vesicle monoamine transporter type 2|vesicle monoamine/H+ antiporter|vesicular amine transporter 2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS006571
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