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Product Info Summary
|Description:||Transient overexpression lysate of WD repeat domain 75 (WDR75)|
WDR75 (NM_032168) Human Over-expression Lysate
Transient overexpression lysate of WD repeat domain 75 (WDR75)
Storage & Handling
Cite This Product
WDR75 (NM_032168) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084128)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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WDR75 Human Over-expression Lysates NM_032168.
Western blot validation of overexpression lysate (LS084128) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with WDR75 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for WDR75 (Source: Uniprot.org, NCBI)
WD repeat-containing protein 75
DKFZp781N1244; FLJ12519; NET16; WD repeat domain 75; WD repeat-containing protein 75 WDR75 NET16, UTP17 WD repeat domain 75 WD repeat-containing protein 75|U3 small nucleolar RNA-associated protein 17 homolog|UTP17, small subunit (SSU) processome component, homolog*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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