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Product Info Summary
|Description:||Transient overexpression lysate of WW and C2 domain containing 2 (WWC2)|
WWC2 (NM_024949) Human Over-expression Lysate
Transient overexpression lysate of WW and C2 domain containing 2 (WWC2)
Storage & Handling
Cite This Product
WWC2 (NM_024949) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080014)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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WWC2 Human Over-expression Lysates NM_024949.
Western blot validation of overexpression lysate (LS080014) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with WWC2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for WWC2 (Source: Uniprot.org, NCBI)
C2 and coiled-coil domain containing 2; FLJ34082; WW and C2 domain containing 2 WWC2 BOMB WW and C2 domain containing 2 protein WWC2|BH3-only member B protein|WW, C2 and coiled-coil domain containing 2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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No publications found for LS080014
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