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Product Info Summary
|Description:||Transient overexpression lysate of chemokine (C motif) ligand 2 (XCL2)|
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XCL2 (NM_003175) Human Over-expression Lysate
Transient overexpression lysate of chemokine (C motif) ligand 2 (XCL2)
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Cite This Product
XCL2 (NM_003175) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006846)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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XCL2 Human Over-expression Lysates NM_003175.
Western blot validation of overexpression lysate (LS006846) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with XCL2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for XCL2 (Source: Uniprot.org, NCBI)
Cytokine SCM-1 beta
intercrine gamma family
Cytokine SCM-1 beta XCL2 SCM-1b, SCM1B, SCYC2 X-C motif chemokine ligand 2 cytokine SCM-1 beta|XC chemokine ligand 2|c motif chemokine 2|chemokine (C motif) ligand 2|small inducible cytokine subfamily C, member 2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on XCL2, check out the XCL2 Infographic
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