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Product Info Summary
|Description:||Transient overexpression lysate of zinc finger protein 91 homolog (mouse) (ZFP91)|
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ZFP91 (NM_053023) Human Over-expression Lysate
Transient overexpression lysate of zinc finger protein 91 homolog (mouse) (ZFP91)
Storage & Handling
Cite This Product
ZFP91 (NM_053023) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080829)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ZFP91 Human Over-expression Lysates NM_053023.
Western blot validation of overexpression lysate (LS080829) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZFP91 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ZFP91 (Source: Uniprot.org, NCBI)
E3 ubiquitin-protein ligase ZFP91
krueppel C2H2-type zinc-finger protein family
FLJ57065; ZFP-91; zinc finger protein 91 homolog (mouse); zinc finger protein homologous to Zfp91 in mouse ZFP91 DMS-8, DSM-8, FKSG11, PZF, ZFP-91, ZNF757 ZFP91 zinc finger protein, atypical E3 ubiquitin ligase E3 ubiquitin-protein ligase ZFP91|RING-type E3 ubiquitin transferase ZFP91|drug resistance-associated sequence in melanoma 8|penta zinc finger protein|zinc finger protein 757|zinc finger protein 91 homolog|zinc finger protein homologous to Zfp91 in mouse*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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