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Product Info Summary
|Description:||Transient overexpression lysate of zinc finger protein 394 (ZNF394)|
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ZNF394 (NM_032164) Human Over-expression Lysate
Transient overexpression lysate of zinc finger protein 394 (ZNF394)
Storage & Handling
Cite This Product
ZNF394 (NM_032164) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084124)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ZNF394 Human Over-expression Lysates NM_032164.
Western blot validation of overexpression lysate (LS084124) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZNF394 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ZNF394 (Source: Uniprot.org, NCBI)
Zinc finger protein 394
krueppel C2H2-type zinc-finger protein family
dJ874C20.2; FLJ23407; zinc finger protein 310 pseudogene; zinc finger protein 323; ZNF20-Lp; ZNF310P ZNF394 ZKSCAN14, ZSCAN46 zinc finger protein 394 zinc finger protein 394|zinc finger protein 99|zinc finger protein with KRAB and SCAN domains 14*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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