A1BG (NM_130786) Human Over-expression Lysate

Lysate of alpha 1B-Glycoprotein over-expression cell lines, WB control

Transient overexpression lysate of alpha-1-B glycoprotein (A1BG)

Product Info Summary

SKU: LS000001
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of alpha-1-B glycoprotein (A1BG)

Product Name

A1BG (NM_130786) Human Over-expression Lysate

See all alpha 1B-Glycoprotein products

SKU/Catalog Number

LS000001

Size

100 µg

Description

Transient overexpression lysate of alpha-1-B glycoprotein (A1BG)

Storage & Handling

Cite This Product

A1BG (NM_130786) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000001)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for A1BG (Source: Uniprot.org, NCBI)

Gene Name

A1BG

Full Name

Alpha-1B-glycoprotein

Weight

54.254kDa

Alternative Names

A1B; A1BG; ABG; alpha 1BGlycoprotein; alpha 1B-Glycoprotein; GAB A1BG A1B, ABG, GAB, HYST2477 alpha-1-B glycoprotein alpha-1B-glycoprotein|HEL-S-163pA|epididymis secretory sperm binding protein Li 163pA

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on A1BG, check out the A1BG Infographic

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