Product Info Summary
SKU: | EKC1716 |
---|---|
Size: | 1 kit, containing one 96-well plate and all necessary reagents |
Reactive Species: | Human, Mouse, Rat |
Application: | ELISA |
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Product info
Product Name
ACV1B Colorimetric Cell-Based ELISA
See all Activin RIB/ALK-4 products
SKU/Catalog Number
EKC1716
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The ACV1B Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ACV1B protein expression profile in cells. The kit can be used for measuring the relative amounts of ACV1B in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ACV1B.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
ACV1B Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1716)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1716 is reactive to ACVR1B in Human, Mouse, Rat samples
Application Guarantee
EKC1716 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-Activin RIB/ALK-4 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 6ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from 293/mouse liver cells
Protein Target Info & Infographic
Gene/Protein Information For ACVR1B (Source: Uniprot.Org, NCBI)
Gene Name
ACVR1B
Full Name
Activin receptor type-1B
Weight
56.807kDa
Superfamily
protein kinase superfamily
Alternative Names
activin A receptor, type IB; Activin receptor type IB; Activin receptor-like kinase 4; Activin RIB; ActivinRIB; ACTRIB; ACVR1B; ACVRLK4ACTR-IB; ALK4; ALK-4; ALK4activin A receptor, type II-like kinase 4; EC 2.7.11; EC 2.7.11.30; Serine/threonine-protein kinase receptor R2; SKR2activin receptor type-1B ACVR1B ACTRIB, ACVRLK4, ALK4, SKR2 activin A receptor type 1B activin receptor type-1B|activin A receptor, type IB|activin A receptor, type II-like kinase 4|activin receptor-like kinase 4|serine/threonine-protein kinase receptor R2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ACVR1B, check out the ACVR1B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ACVR1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ACV1B Colorimetric Cell-Based ELISA (EKC1716)
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