Product Info Summary
SKU: | LS010939 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of AFG3 ATPase family gene 3-like 2 (yeast) (AFG3L2), nuclear gene encoding mitochondrial protein |
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Product info
Product Name
AFG3L2 (NM_006796) Human Over-expression Lysate
SKU/Catalog Number
LS010939
Size
100 µg
Description
Transient overexpression lysate of AFG3 ATPase family gene 3-like 2 (yeast) (AFG3L2), nuclear gene encoding mitochondrial protein
Storage & Handling
Cite This Product
AFG3L2 (NM_006796) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010939)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
AFG3L2 Human Over-expression Lysates NM_006796.
Western blot validation of overexpression lysate (LS010939) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AFG3L2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for AFG3L2 (Source: Uniprot.org, NCBI)
Gene Name
AFG3L2
Full Name
AFG3-like protein 2
Weight
88.584kDa
Alternative Names
AFG3 (ATPase family gene 3, yeast)-like 2; AFG3 ATPase family gene 3-like 2 (S. cerevisiae); AFG3 ATPase family gene 3-like 2 (yeast); AFG3-like protein 2; ATPase family gene 3, yeast; EC 3.4.24; EC 3.4.24.-; FLJ25993; Paraplegin-like protein; SCA28; spinocerebellar ataxia 28 AFG3L2 OPA12, SCA28, SPAX5 AFG3 like matrix AAA peptidase subunit 2 AFG3-like protein 2|AFG3 ATPase family gene 3-like 2|AFG3 ATPase family member 3-like 2|AFG3 like AAA ATPase 2|ATPase family gene 3, yeast|paraplegin-like protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on AFG3L2, check out the AFG3L2 Infographic
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In this infographic, you will see the following information for AFG3L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AFG3L2 (NM_006796) Human Over-expression Lysate (LS010939)
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No publications found for LS010939
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