AIF (AIFM1) (NM_004208) Human Over-expression Lysate

Product Name

AIF (AIFM1) (NM_004208) Human Over-expression Lysate

See all AIF products

SKU/Catalog Number

LS009131

Size

100 µg

Description

Transient overexpression lysate of apoptosis-inducing factor, mitochondrion-associated, 1 (AIFM1), nuclear gene encoding mitochondrial protein, transcript variant 1

Storage & Handling

Cite This Product

AIF (AIFM1) (NM_004208) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009131)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for AIFM1 (Source: Uniprot.org, NCBI)

Alternative Names

AIF; AIFapoptosis-inducing factor 1, mitochondrial; AIFM1; apoptosis-inducing factor, mitochondrion-associated, 1; COXPD6; MGC111425; PDCD8; PDCD8striatal apoptosis-inducing factor; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8 AIFM1 AIF, AUNX1, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, SEMDHL apoptosis inducing factor mitochondria associated 1 apoptosis-inducing factor 1, mitochondrial|apoptosis-inducing factor, mitochondrion-associated, 1|auditory neuropathy, X-linked recessive 1|programmed cell death 8 (apoptosis-inducing factor)|striatal apoptosis-inducing factor|testicular secretory protein Li 4

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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