Product Info Summary
SKU: | LS057016 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10) |
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Product info
Product Name
AKR1B10 (NM_020299) Human Over-expression Lysate
See all Aldo-keto Reductase 1B10/AKR1B10 products
SKU/Catalog Number
LS057016
Size
100 µg
Description
Transient overexpression lysate of aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10)
Storage & Handling
Cite This Product
AKR1B10 (NM_020299) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS057016)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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AKR1B10 Human Over-expression Lysates NM_020299.
Western blot validation of overexpression lysate (LS057016) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AKR1B10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for AKR1B10 (Source: Uniprot.org, NCBI)
Gene Name
AKR1B10
Full Name
Aldo-keto reductase family 1 member B10
Weight
36.02kDa
Superfamily
aldo/keto reductase family
Alternative Names
AKR1B10; AKR1B11; AKR1B12; Aldo-keto Reductase 1B10; aldo-keto reductase family 1 member B10; aldo-keto reductase family 1, member B10 (aldose reductase); aldo-keto reductase family 1, member B11 (aldose reductase-like); AldoketoReductase 1B10; aldose reductase-like 1; aldose reductase-like peptide; Aldose reductase-like; Aldose reductase-related protein; ALDRLn; ARL1; ARL-1SI reductase; ARP; EC 1.1.1; EC 1.1.1.-; EC 1.1.1.21; hARP; HIS; HSI; MGC14103; Small intestine reductase AKR1B10 AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HSI aldo-keto reductase family 1 member B10 aldo-keto reductase family 1 member B10|ARP|SI reductase|aldo-keto reductase family 1, member B10 (aldose reductase)|aldo-keto reductase family 1, member B11 (aldose reductase-like)|aldose reductase-like 1|aldose reductase-like peptide|aldose reductase-related protein|hARP|small intestine reductase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on AKR1B10, check out the AKR1B10 Infographic
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In this infographic, you will see the following information for AKR1B10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AKR1B10 (NM_020299) Human Over-expression Lysate (LS057016)
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