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Product Info Summary
| SKU: | LS054890 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of alkB, alkylation repair homolog 5 (E. coli) (ALKBH5) |
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Product info
Product Name
ALKBH5 (NM_017758) Human Over-expression Lysate
SKU/Catalog Number
LS054890
Size
100 µg
Description
Transient overexpression lysate of alkB, alkylation repair homolog 5 (E. coli) (ALKBH5)
Storage & Handling
Cite This Product
ALKBH5 (NM_017758) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS054890)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ALKBH5 Human Over-expression Lysates NM_017758.
Western blot validation of overexpression lysate (LS054890) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ALKBH5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ALKBH5 (Source: Uniprot.org, NCBI)
Gene Name
ALKBH5
Full Name
RNA demethylase ALKBH5
Weight
44.256kDa
Superfamily
alkB family
Alternative Names
ABH5OFOXD; alkB, alkylation repair homolog 5 (E. coli); Alkylated DNA repair protein alkB homolog 5; EC 1.14.11.-; FLJ20308; OFOXD1; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5 ALKBH5 ABH5, OFOXD, OFOXD1 alkB homolog 5, RNA demethylase RNA demethylase ALKBH5|AlkB family member 5, RNA demethylase|alkB, alkylation repair homolog 5|alkylated DNA repair protein alkB homolog 5|alpha-ketoglutarate-dependent dioxygenase alkB homolog 5|oxoglutarate and iron-dependent oxygenase domain containing|probable alpha-ketoglutarate-dependent dioxygenase ABH5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ALKBH5, check out the ALKBH5 Infographic
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In this infographic, you will see the following information for ALKBH5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ALKBH5 (NM_017758) Human Over-expression Lysate (LS054890)
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