RNA demethylase ALKBH5 Antibodies

AND ALKBH5 ELISA kits, RNA demethylase ALKBH5 Proteins

Many biological assays use antibodies to detect RNA demethylase ALKBH5, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RNA demethylase ALKBH5 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RNA demethylase ALKBH5 antibodies...

We validate the specificity of these antibodies to RNA demethylase ALKBH5 by testing them on tissues known to express ALKBH5 positively and negatively. Browse below to find the ALKBH5 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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ALKBH5 Infographic by Boster Bio

All ALKBH5 Products

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Anti-ALKBH5 Antibody Picoband®

4 Citations

Western blot analysis of ALKBH5 using anti-ALKBH5 antibody (A03360)

IF analysis of ALKBH5 using anti-ALKBH5 antibody (A03360) and anti-Tubulin Alpha antibody (M03989-3)

Human

ELISA, IF, ICC, WB

$370
Cat # A03360
100 μg/vial

Anti-RNA demethylase ALKBH5 ALKBH5 Antibody

Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night

Immunohistochemical analysis of paraffin-embedded human tonsil

Human, Mouse

IF, IHC, WB

$380
Cat # A03360-2
100ul

ALKBH5 (NM_017758) Human Over-expression Lysate

Human

$628
Cat # LS054890
20 µg, 100 µg

Anti-ALKBH5 Antibody (Monoclonal, 34A44)

Human, Mouse, Rat

IHC, WB

$370
Cat # M03360
100 μl/vial

ALKBH5 Overview

Facts about RNA demethylase ALKBH5.

ALKBH5 3D structure. Source: alphafold

RNA demethylase ALKBH5 (ALKBH5)

Dioxygenase that demethylates RNA by oxidative demethylation: namely demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N(6)- methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105).

Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736).

Gene Information

Human
Gene Name:	ALKBH5
Uniprot:	Q6P6C2
Entrez:	54890

Family

Belongs to:

alkB family

Alternative Names

ABH5OFOXD; alkB, alkylation repair homolog 5 (E. coli); Alkylated DNA repair protein alkB homolog 5; EC 1.14.11.-; FLJ20308; OFOXD1; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5

Molecular Weight

Mass (kDA):
44.256 kDA

Genomic Context


Human

Location:	17p11.2\|17p11.2
Sequence:	17; NC_000017.11 (18183828..18209954)

Tissue Specificity

Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.

Subcellular Localizations

Nucleus speckle.

Diseases

Hypoxia
Anoxia

Pathways

Methylation
Regulation Of Dna Repair
Dna Repair
Chromatin Remodeling
Demethylation

PTMs

Decarboxylation

Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ALKBH5

References

PMID: 17979886 by Tsujikawa K., et al. Expression and sub-cellular localization of human ABH family molecules.

PMID: 21264265 by Thalhammer A., et al. Human AlkB homologue 5 is a nuclear 2-oxoglutarate dependent oxygenase and a direct target of hypoxia-inducible factor 1alpha (HIF- 1alpha).

1 Publications of Boster RNA demethylase ALKBH5 Antibodies & ELISA Kits

PubMed ID: 10.1002/smtd.202001045, RNA m6A Modification Alteration by Black Phosphorus Quantum Dots Regulates Cell ...

*More publications can be found for each product on its corresponding product page