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Product Info Summary
| SKU: | LS000118 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of adducin 1 (alpha) (ADD1), transcript variant 1 |
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Product info
Product Name
alpha Adducin (ADD1) (NM_001119) Human Over-expression Lysate
See all alpha Adducin products
SKU/Catalog Number
LS000118
Size
100 µg
Description
Transient overexpression lysate of adducin 1 (alpha) (ADD1), transcript variant 1
Storage & Handling
Cite This Product
alpha Adducin (ADD1) (NM_001119) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000118)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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alpha Adducin (ADD1) Human Over-expression Lysates NM_001119.
Western blot validation of overexpression lysate (LS000118) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ADD1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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alpha Adducin (ADD1) Human Over-expression Lysates NM_014190.
Western blot validation of overexpression lysate (LS000118) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ADD1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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alpha Adducin (ADD1) Human Over-expression Lysates NM_176801.
Western blot validation of overexpression lysate (LS000118) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ADD1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ADD1 (Source: Uniprot.org, NCBI)
Gene Name
ADD1
Full Name
Alpha-adducin
Weight
80.955kDa
Superfamily
aldolase class II family
Alternative Names
ADDA; adducin 1 (alpha); alpha-adducin; erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427 ADD1 ADDA adducin 1 alpha-adducin|adducin 1 (alpha)|erythrocyte adducin alpha subunit
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ADD1, check out the ADD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For alpha Adducin (ADD1) (NM_001119) Human Over-expression Lysate (LS000118)
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