AML1 (Phospho-Ser303) Colorimetric Cell-Based ELISA Kit

RUNX1/CBFA2 ELISA kit, phospho-specific, cell-based

The AML1 (Phospho-Ser303) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AML1 (Phospho-Ser303) protein expression profile in cells. The kit can be used for measuring the relative amounts of AML1 (Phospho-Ser303) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AML1 (Phospho-Ser303).

Product Info Summary

SKU: EKC2017
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

AML1 (Phospho-Ser303) Colorimetric Cell-Based ELISA Kit

See all RUNX1/CBFA2 products

SKU/Catalog Number

EKC2017

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The AML1 (Phospho-Ser303) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AML1 (Phospho-Ser303) protein expression profile in cells. The kit can be used for measuring the relative amounts of AML1 (Phospho-Ser303) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AML1 (Phospho-Ser303).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

AML1 (Phospho-Ser303) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2017)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2017 is reactive to RUNX1 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2017 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-AML1 Phospho-Ser303 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-AML1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For RUNX1 (Source: Uniprot.Org, NCBI)

Gene Name

RUNX1

Full Name

Runt-related transcription factor 1

Weight

48737 MW

Alternative Names

Acute myeloid leukemia 1 protein;CBF-alpha 2;CBFA2;Core-binding factor alpha 2 subunit;Oncogene AML-1;PEA2-alpha B;PEBP2-alpha B;Polyomavirus enhancer binding protein 2 alpha B subunit;RUN1;Runt-related transcription factor 1;RUNX1;SL3-3 enhancer factor 1 alpha B subunit;SL3/AKV core-binding factor alpha B subunit RUNX1 AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha RUNX family transcription factor 1 runt-related transcription factor 1|AML1-EVI-1 fusion protein|PEA2-alpha B|PEBP2-alpha B|SL3-3 enhancer factor 1 alpha B subunit|SL3/AKV core-binding factor alpha B subunit|acute myeloid leukemia 1 protein|core-binding factor, runt domain, alpha subunit 2|mutant RUNX1|oncogene AML-1|polyomavirus enhancer-binding protein 2 alpha B subunit|runt related transcription factor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RUNX1, check out the RUNX1 Infographic

RUNX1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RUNX1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EKC2017

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used AML1 (Phospho-Ser303) Colorimetric Cell-Based ELISA Kit?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For AML1 (Phospho-Ser303) Colorimetric Cell-Based ELISA Kit

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for AML1 (Phospho-Ser303) Colorimetric Cell-Based ELISA Kit

$540
Backordered.

Lead time for this item is typically 5-7 days

Get A Quote
In stock
Order Product
EKC2017
$540.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.