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Product Info Summary
| SKU: | PB9157 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, IHC-F, WB |
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Product info
Product Name
Anti-RUNX1/AML1 Antibody Picoband®
SKU/Catalog Number
PB9157
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RUNX1/AML1 Antibody Picoband® catalog # PB9157. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RUNX1/AML1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9157)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human RUNX1, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9157 is reactive to RUNX1 in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
48.7 kDa
Background of RUNX1
Runt-related transcription factor 1 (RUNX1), also known as AML1 or CBFA2, is a protein that in humans is encoded by the RUNX1 gene. It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). RUNX1 is mapped to 21q22.12. RUNX1 is a transcription factor that regulates the differentiation of hematopoietic stem cells into mature blood cells. RUNX proteins form a heterodimeric complex with CBFβ which confers increased DNA binding and stability to the complex. Chromosomal translocations involving the RUNX1 gene are associated with several types of leukemia including M2 AML. Mutations in RUNX1 are implicated in cases of breast cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9157 is guaranteed for IHC, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat
Positive Control
WB: human HL-60 whole cell, rat thymus tissue, mouse thymus tissue,
IHC: rat thymus tissue, human mammary cancer tissue, mouse intestine tissue, rat intestine tissue
IHC-F: rat spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of RUNX1 using anti-RUNX1 antibody (PB9157).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse thymus tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1 antigen affinity purified polyclonal antibody (Catalog # PB9157) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX1 at approximately 55KD. The expected band size for RUNX1 is at 49KD.
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IHC analysis of RUNX1 using anti-RUNX1 antibody (PB9157).
RUNX1 was detected in paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX1 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of RUNX2 using anti-RUNX2 antibody (PB9157).
RUNX2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX2 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9157).
RUNX3 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX3 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9157).
RUNX3 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX3 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9157).
RUNX3 was detected in frozen section of rat spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX3 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-RUNX1/AML1 Antibody Picoband® (PB9157)
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Customer Q&As
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6 Customer Q&As for Anti-RUNX1/AML1 Antibody Picoband®
Question
We are currently using anti-RUNX1/AML1 antibody PB9157 for mouse tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The anti-RUNX1/AML1 antibody (PB9157) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18
Question
We purchased anti-RUNX1/AML1 antibody for IHC-F on erythroleukemia last year. I am using rat, and I plan to use the antibody for WB next. I am interested in examining erythroleukemia as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
I have checked the website and datasheets of our anti-RUNX1/AML1 antibody and it appears that PB9157 has been validated on rat in both IHC-F and WB. Thus PB9157 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-05
Question
We were happy with the WB result of your anti-RUNX1/AML1 antibody. However we have seen positive staining in leukemic t-cell nucleus. using this antibody. Is that expected? Could you tell me where is RUNX1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
According to literature, leukemic t-cell does express RUNX1. Generally RUNX1 expresses in nucleus. Regarding which tissues have RUNX1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7891692
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Leukocyte, Pubmed ID: 1720541
Liver, Pubmed ID: 24275569
Monocyte, Pubmed ID: 7835892, 8634147
Peripheral blood, Pubmed ID: 8395054
Boster Scientific Support
Answered: 2019-05-14
Question
For the PB9157 RUNX1 antibody, the customer wants to know if this antibody has been tested to detect endogenous RUNX1 protein with human and mouse samples. Do you have any information?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
PB9157 has been tested to detect endogenous RUNX1 protein with human and mouse samples. Please see the images on the product page. https://www.bosterbio.com/anti-runx1-aml1-picoband-trade-antibody-pb9157-boster.html?options=cart
Boster Scientific Support
Answered: 2019-02-08
Question
We have seen staining in rat cervix carcinoma. What should we do? Is anti-RUNX1/AML1 antibody supposed to stain cervix carcinoma positively?
G. Banerjee
Verified customer
Asked: 2017-07-17
Answer
According to literature cervix carcinoma does express RUNX1. According to Uniprot.org, RUNX1 is expressed in epithelium of mammary gland, leukocyte, monocyte, b-cell, cervix carcinoma, leukemic t-cell, erythroleukemia, liver, peripheral blood, among other tissues. Regarding which tissues have RUNX1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7891692
Cervix carcinoma, Pubmed ID: 18669648
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Leukocyte, Pubmed ID: 1720541
Liver, Pubmed ID: 24275569
Monocyte, Pubmed ID: 7835892, 8634147
Peripheral blood, Pubmed ID: 8395054
Boster Scientific Support
Answered: 2017-07-17
Question
We need using your anti-RUNX1/AML1 antibody for negative regulation of transcription by rna polymerase ii studies. Has this antibody been tested with western blotting on intestine tissue? We would like to see some validation images before ordering.
P. Parker
Verified customer
Asked: 2017-01-30
Answer
Thanks for your inquiry. This PB9157 anti-RUNX1/AML1 antibody is tested on rat thymus tissue, mouse thymus tissue, mammary cancer tissue, intestine tissue, spleen tissue. It is guaranteed to work for IHC-P, IHC-F, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-01-30
