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Product Info Summary
| SKU: | M04148 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-14-3-3 gamma YWHAG Rabbit Monoclonal Antibody
SKU/Catalog Number
M04148
BM4688 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-14-3-3 gamma YWHAG Rabbit Monoclonal Antibody catalog # M04148. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 gamma YWHAG Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04148)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HAH-25
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human 14-3-3 gamma
Reactive Species
M04148 is reactive to YWHAG in Human, Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
28.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04148 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
FC 1:20
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of 14-3-3 gamma expression in HeLa cell lysate.
Specific Publications For Anti-14-3-3 gamma YWHAG Rabbit Monoclonal Antibody (M04148)
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Customer Q&As
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3 Customer Q&As for Anti-14-3-3 gamma YWHAG Rabbit Monoclonal Antibody
Question
I see that the anti-14-3-3 gamma Rabbit Monoclonal antibody M04148 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-05
Question
We are currently using anti-14-3-3 gamma Rabbit Monoclonal antibody M04148 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Z. Zhao
Verified customer
Asked: 2016-05-13
Answer
The anti-14-3-3 gamma Rabbit Monoclonal antibody (M04148) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-13
Question
Is there a BSA free version of anti-14-3-3 gamma Rabbit Monoclonal antibody M04148 available?
A. Singh
Verified customer
Asked: 2014-10-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-14-3-3 gamma Rabbit Monoclonal antibody M04148 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-10-24
