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Product Info Summary
| SKU: | M03904 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-14-3-3 Theta YWHAQ Rabbit Monoclonal Antibody
SKU/Catalog Number
M03904
BM4757 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-14-3-3 Theta YWHAQ Rabbit Monoclonal Antibody catalog # M03904. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 Theta YWHAQ Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03904)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HHF-25
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human 14-3-3 Theta
Reactive Species
M03904 is reactive to YWHAQ in Human, Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
27.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03904 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: human Hela whole cell, human A549 whole cell, human SH-SY5Y whole cell, human U251 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue
IHC: human pancreas cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of YWHAQ using anti-YWHAQ antibody (M03904).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAQ antigen affinity purified monoclonal antibody (M03904) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for YWHAQ at approximately 28 kDa. The expected band size for YWHAQ is at 28 kDa.
Click image to see more details
IHC analysis of YWHAQ using anti-YWHAQ antibody (M03904).
YWHAQ was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-YWHAQ Antibody (M03904) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-14-3-3 Theta YWHAQ Rabbit Monoclonal Antibody (M03904)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-14-3-3 Theta YWHAQ Rabbit Monoclonal Antibody
Question
I see that the anti-14-3-3 Theta Rabbit Monoclonal antibody M03904 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-14-3-3 Theta Rabbit Monoclonal antibody M03904 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-14-3-3 Theta Rabbit Monoclonal antibody (M03904) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
We ordered your anti-14-3-3 Theta Rabbit Monoclonal antibody for IF on liver in a previous project. I am using human, and We intend to use the antibody for Flow Cytometry next. I was wanting to use examining liver as well as t-cell in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
I have checked the website and datasheets of our anti-14-3-3 Theta Rabbit Monoclonal antibody and it appears that M03904 has been validated on human in both IF and Flow Cytometry. Thus M03904 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-27
Question
We have seen staining in human t-cell. Do you have any suggestions? Is anti-14-3-3 Theta Rabbit Monoclonal antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
From literature t-cell does express YWHAQ. From Uniprot.org, YWHAQ is expressed in substantia nigra, t-cell, keratinocyte, placenta, skin uterus, platelet, b-cell lymphoma hepatoma, brain, lymphoblast, cervix carcinoma, leukemic t-cell, liver, among other tissues. Regarding which tissues have YWHAQ expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9360956
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Keratinocyte, Pubmed ID: 8515476
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Placenta, Skin, and Uterus, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
T-cell, Pubmed ID: 2015305
Boster Scientific Support
Answered: 2020-02-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-14-3-3 Theta Rabbit Monoclonal antibody M03904. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-05
Question
Can you help my question with product M03904, anti-14-3-3 Theta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03904 anti-14-3-3 Theta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-23
Question
Is a blocking peptide available for product anti-14-3-3 Theta Rabbit Monoclonal antibody (M03904)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We do provide the blocking peptide for product anti-14-3-3 Theta Rabbit Monoclonal antibody (M03904). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-27
Question
Will anti-14-3-3 Theta Rabbit Monoclonal antibody M03904 work for ICC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-03-27
Answer
According to the expression profile of leukemic t-cell, YWHAQ is highly expressed in leukemic t-cell. So, it is likely that anti-14-3-3 Theta Rabbit Monoclonal antibody M03904 will work for ICC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-03-27
Question
We want to test anti-14-3-3 Theta Rabbit Monoclonal antibody M03904 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-14-3-3 Theta Rabbit Monoclonal antibody M03904 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
The products we sell, including anti-14-3-3 Theta Rabbit Monoclonal antibody M03904, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-31
Question
Would M03904 anti-14-3-3 Theta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
As indicated on the product datasheet, M03904 anti-14-3-3 Theta Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-16
Question
Is there a BSA free version of anti-14-3-3 Theta Rabbit Monoclonal antibody M03904 available?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-14-3-3 Theta Rabbit Monoclonal antibody M03904 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-01
Question
Is this M03904 anti-14-3-3 Theta Rabbit Monoclonal antibody reactive to the isotypes of YWHAQ?
K. Rodriguez
Verified customer
Asked: 2017-05-29
Answer
The immunogen of M03904 anti-14-3-3 Theta Rabbit Monoclonal antibody is A synthesized peptide derived from human 14-3-3 Theta . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-29
Question
I was wanting to use your anti-14-3-3 Theta Rabbit Monoclonal antibody for ICC for mouse leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse leukemic t-cell identification?
K. Li
Verified customer
Asked: 2017-01-25
Answer
It shows on the product datasheet, M03904 anti-14-3-3 Theta Rabbit Monoclonal antibody has been validated for Flow Cytometry, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-25
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-14-3-3 Theta Rabbit Monoclonal antibody M03904. Please let me know if you require anything else.
Z. Dhar
Verified customer
Asked: 2015-10-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-01
Question
Our team were happy with the WB result of your anti-14-3-3 Theta Rabbit Monoclonal antibody. However we have observed positive staining in placenta axonally using this antibody. Is that expected? Could you tell me where is YWHAQ supposed to be expressed?
T. Johnson
Verified customer
Asked: 2013-02-14
Answer
From what I have seen in literature, placenta does express YWHAQ. Generally YWHAQ expresses in cytoplasm. note=in neurons, axonally. Regarding which tissues have YWHAQ expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9360956
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Keratinocyte, Pubmed ID: 8515476
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Placenta, Skin, and Uterus, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
T-cell, Pubmed ID: 2015305
Boster Scientific Support
Answered: 2013-02-14
