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Product Info Summary
| SKU: | A06032-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-4-1BBL/Tnfsf9 Antibody Picoband®
SKU/Catalog Number
A06032-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-4-1BBL/Tnfsf9 Antibody Picoband® catalog # A06032-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-4-1BBL/Tnfsf9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06032-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse 4-1BBL/Tnfsf9 recombinant protein (Position: M1-E309).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06032-2 is reactive to Tnfsf9 in Mouse, Rat
Observed Molecular Weight
27 kDa
Calculated molecular weight
33.9 kDa
Background of Tnfsf9
Tumor necrosis factor ligand superfamily member 9 also known as 4-1BB ligand or 4-1BBL or CD137L is a protein that in humans is encoded by the TNFSF9 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06032-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, rat lung tissue, rat C6 whole cell, mouse brain tissue, mouse thymus tissue, mouse lung tissue, mouse RAW2647 whole cell
FCM: ANA-1 cell
Validation Images & Assay Conditions
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Western blot analysis of 4-1BBL/Tnfsf9 using anti-4-1BBL/Tnfsf9 antibody (A06032-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-4-1BBL/Tnfsf9 antigen affinity purified polyclonal antibody (Catalog # A06032-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 4-1BBL/Tnfsf9 at approximately 27 kDa. The expected band size for 4-1BBL/Tnfsf9 is at 27 kDa.
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Flow Cytometry analysis of ANA-1 cells using anti-4-1BBL/Tnfsf9 antibody (A06032-2).
Overlay histogram showing ANA-1 cells stained with A06032-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-4-1BBL/Tnfsf9 Antibody (A06032-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-4-1BBL/Tnfsf9 Antibody Picoband® (A06032-2)
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