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Product Info Summary
| SKU: | A03718 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-4.1G Antibody
SKU/Catalog Number
A03718
Size
100μl
Form
Liquid
Description
Boster Bio Anti-4.1G Antibody catalog # A03718. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-4.1G Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03718)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from 4.1G . at AA range: 530-610
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03718 is reactive to EPB41L2 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
112.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03718 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Western Blot analysis of K562 cells using 4.1G Polyclonal Antibody
Specific Publications For Anti-4.1G Antibody (A03718)
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Customer Q&As
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6 Customer Q&As for Anti-4.1G Antibody
Question
Is a blocking peptide available for product anti-4.1G antibody (A03718)?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
We do provide the blocking peptide for product anti-4.1G antibody (A03718). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-04
Question
My question regarding product A03718, anti-4.1G antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03718 anti-4.1G antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-04
Question
My question regards to test anti-4.1G antibody A03718 on human brain heart for research purposes, then I may be interested in using anti-4.1G antibody A03718 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The products we sell, including anti-4.1G antibody A03718, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-24
Question
Will anti-4.1G antibody A03718 work on pig WB with ovarian carcinoma pre-b cell?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Our lab technicians have not validated anti-4.1G antibody A03718 on pig. You can run a BLAST between pig and the immunogen sequence of anti-4.1G antibody A03718 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig ovarian carcinoma pre-b cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
We are currently using anti-4.1G antibody A03718 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
G. Miller
Verified customer
Asked: 2016-07-19
Answer
The anti-4.1G antibody (A03718) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-19
Question
Do you have a BSA free version of anti-4.1G antibody A03718 available?
A. Krishna
Verified customer
Asked: 2014-08-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-4.1G antibody A03718 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-08-18
