Product Info Summary
| SKU: | A01691 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-40S ribosomal protein SA RPSA Antibody
SKU/Catalog Number
A01691
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-40S ribosomal protein SA RPSA Antibody (Catalog # A01691). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
RPSA antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-40S ribosomal protein SA RPSA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01691)
Host
Rabbit
Contents
RPSA antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RPSA antibody was raised against a 17 amino acid peptide near the carboxy terminus of human RPSA. The immunogen is located within amino acids 190 - 240 of RPSA.
Cross-reactivity
RPSA antibody is human, mouse and rat reactive.
Reactive Species
A01691 is reactive to RPSA in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
32.9 kDa
Background of RPSA
The 40S ribosomal protein SA (RPSA) is a high-affinity, non-integrin family, laminin receptor, also known as 67 kDa laminin receptor or 37 kDa laminin receptor precursor (37LRP) (1). Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes and have been implicated in a variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis (2). RPSA is overexpressed in multiple types of cancers and has recently been shown to act as a cancer-specific death receptor (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01691 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RPSA antibody can be used for detection of RPSA by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RPSA in Caco-2 cell lysate with RPSA antibody at 1 μg/ml.
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Immunohistochemistry of RPSA in mouse liver tissue with RPSA antibody at 5 μg/mL.
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Immunofluorescence of RPSA in mouse liver tissue with RPSA antibody at 20 μg/mL.
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Immunohistochemistry of RPSA in mouse brain tissue with RPSA Antibody at 5 μg/mL.
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Immunofluorescence of RPSA in mouse brain tissue with RPSA Antibody at 20 μg/mL.
Green: RPSA antibody (A01691)
Red: Phylloidin staining
Blue: DAPI staining
Specific Publications For Anti-40S ribosomal protein SA RPSA Antibody (A01691)
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