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- Table of Contents
TP53BP1 antibody (M00397) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of 53BP1 using anti-53BP1 antibody (M00397).
53BP1 was detected in a paraffin-embedded section of human acinic cell carcinoma of parotid tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-53BP1 Antibody (M00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
53BP1 was detected in a paraffin-embedded section of human acinic cell carcinoma of parotid tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-53BP1 Antibody (M00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-53BP1 TP53BP1 Rabbit Monoclonal Antibody
TP53BP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
TP53BP1 antibody was positively detected by WB in:
human Hacat whole cell, human U251 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TP53BP1 antibody was positively detected by IHC in:
human acinic cell carcinoma of parotid tissue, human acinic cell carcinoma of parotid tissue, human cervical cancer tissue, human cervical cancer tissue, human clear cell renal cell carcinoma tissue, human clear cell renal cell carcinoma tissue, human colon adenocarcinoma tissue, human colon adenocarcinoma tissue, human liver cancer tissue, human liver cancer tissue, human ovarian cancer tissue, human ovarian cancer tissue, human placenta tissue, human placenta tissue, human tonsil tissue, human tonsil tissue, mouse liver tissue, mouse liver tissue, rat liver tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TP53BP1 antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Product Information
Product Name
Anti-53BP1 TP53BP1 Rabbit Monoclonal Antibody
View all TP53BP1 Antibodies
View all TP53BP1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABAI-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human 53BP1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
450 kDa
Calculated Molecular Weight
213.6 kDa
Cite This Product
Anti-53BP1 TP53BP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00397)
Protocols
Western Blot protocol for TP53BP1 antibody
Immunohistochemistry (IHC) protocol for TP53BP1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TP53BP1 antibody
Publications
Application:
Species:
Exploring the Role of Nesprin1 in isoproterenol-induced heart failure: Insights into pathogenesis and therapeutic implications
Published: 2026-08-11
Journal: PLoS One
Application: WB,IF
Impact Factor: 2.80
Species: Mouse
PMID: 42579703
Review
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Product Promise
We promise Anti-53BP1 TP53BP1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00397
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026