Anti-5HT2B Receptor/HTR2B Antibody Picoband®

Product Name

Anti-5HT2B Receptor/HTR2B Antibody Picoband®

View all HTR2B Antibodies

SKU/Catalog Number

PB9600
PB0600 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Serotonin signaling (putative): HTR2B (Gq/PLCβ→IP₃/Ca²⁺, MAPK) in vascular/valvular biology and fibrosis research. Assay: rabbit polyclonal for WB (human/rat). Can be analyzed alongside neutrophil protease activity via Elastase ELISA and anti-angiogenic axis sVEGFR1/sFLT1 ELISA to relate GPCR signaling to vascular remodeling (putative).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-5HT2B Receptor/HTR2B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9600)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT2B Receptor, different from the related mouse sequence by six amino acids, and from the related rat sequence by nine amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9600 is reactive to HTR2B in Human, Rat

Observed Molecular Weight

54 kDa

Calculated molecular weight

54.3 kDa

Background of HTR2B

5HT2B Receptor is known as HTR2B. This gene encodes one of the several different receptors for 5-hydroxytryptamine (serotonin) that belongs to the G-protein coupled receptor 1 family. Serotonin is a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Serotonin receptors mediate many of the central and peripheral physiologic functions of serotonin, including regulation of cardiovascular functions and impulsive behavior. Population and family-based analyses of a minor allele (glutamine-to-stop substitution, designated Q20*) which blocks expression of this protein, and knockout studies in mice, suggest a role for this gene in impulsivity. However, other factors, such as elevated testosterone levels, may also be involved.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9600 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: Rat Brain Tissue, U87 Whole Cell

Validation Images & Assay Conditions

See full target information HTR2B

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