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Product Info Summary
| SKU: | A04197 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-A Cyclase V/VI ADCY5 Antibody
SKU/Catalog Number
A04197
Size
100μl
Form
Liquid
Description
Boster Bio Anti-A Cyclase V/VI ADCY5 Antibody catalog # A04197. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-A Cyclase V/VI ADCY5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04197)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ADCY5/6. AA range:931-980
Reactive Species
A04197 is reactive to ADCY5 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
138.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04197 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of COLO205 cells using A Cyclase V/VI Polyclonal Antibody
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Western blot analysis of lysates from COLO205 cells, using ADCY5/6 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HUVECcells using Collagen V α1 antibody.
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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-A Cyclase V/VI ADCY5 Antibody (A04197)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-A Cyclase V/VI ADCY5 Antibody
Question
Is this A04197 anti-A Cyclase V/VI antibody reactive to the isotypes of ADCY5?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The immunogen of A04197 anti-A Cyclase V/VI antibody is Synthesized peptide derived from the C-terminal region of human A Cyclase V/VI. at AA rangle: 900-980. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-29
Question
We are currently using anti-A Cyclase V/VI antibody A04197 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The anti-A Cyclase V/VI antibody (A04197) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-31
Question
Does A04197 anti-A Cyclase V/VI antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
You can see on the product datasheet, A04197 anti-A Cyclase V/VI antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-A Cyclase V/VI antibody A04197. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-15
Question
Is a blocking peptide available for product anti-A Cyclase V/VI antibody (A04197)?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
We do provide the blocking peptide for product anti-A Cyclase V/VI antibody (A04197). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-30
Question
I was wanting to use your anti-A Cyclase V/VI antibody for WB for rat heart on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat heart identification?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
You can see on the product datasheet, A04197 anti-A Cyclase V/VI antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-04
Question
Would anti-A Cyclase V/VI antibody A04197 work for WB with heart?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
According to the expression profile of heart, ADCY5 is highly expressed in heart. So, it is likely that anti-A Cyclase V/VI antibody A04197 will work for WB with heart.
Boster Scientific Support
Answered: 2019-05-14
Question
My question regarding product A04197, anti-A Cyclase V/VI antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04197 anti-A Cyclase V/VI antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-14
Question
I am looking for to test anti-A Cyclase V/VI antibody A04197 on rat heart for research purposes, then I may be interested in using anti-A Cyclase V/VI antibody A04197 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Parker
Verified customer
Asked: 2018-06-29
Answer
The products we sell, including anti-A Cyclase V/VI antibody A04197, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-29
Question
Here is the WB image, lot number and protocol we used for heart using anti-A Cyclase V/VI antibody A04197. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-27
Question
I see that the anti-A Cyclase V/VI antibody A04197 works with WB, what is the protocol used to produce the result images on the product page?
F. Kulkarni
Verified customer
Asked: 2015-09-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-09-08
Question
Is there a BSA free version of anti-A Cyclase V/VI antibody A04197 available?
N. Li
Verified customer
Asked: 2014-03-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-A Cyclase V/VI antibody A04197 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-03-06
Question
Would anti-A Cyclase V/VI antibody A04197 work on goat WB with heart?
V. Williams
Verified customer
Asked: 2013-04-03
Answer
Our lab technicians have not validated anti-A Cyclase V/VI antibody A04197 on goat. You can run a BLAST between goat and the immunogen sequence of anti-A Cyclase V/VI antibody A04197 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat heart in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-03
