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Product Info Summary
| SKU: | A07362 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-A-kinase anchor protein 4 AKAP4 Antibody
SKU/Catalog Number
A07362
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-A-kinase anchor protein 4 AKAP4 Antibody (Catalog # A07362). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
AKAP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-A-kinase anchor protein 4 AKAP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07362)
Host
Rabbit
Contents
AKAP4 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
AKAP4 antibody was raised against a 17 amino acid peptide near the amino terminus of human AKAP4. The immunogen is located within the first 50 amino acids of AKAP4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.
Reactive Species
A07362 is reactive to AKAP4 in Human
Applications
A07362 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
94.477kDa
Background of AKAP4
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell (1). AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm (2). It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways (2). AKAP4 has also been postulated to be a potential biomarker for breast cancer (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AKAP4 antibody can be used for detection of AKAP4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AKAP4 in human testis tissue lysate with AKAP4 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of AKAP4 in human testis tissue with AKAP4 antibody at 5 μg/ml.
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Immunofluorescence of AKAP4 in human testis tissue with AKAP4 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For AKAP4 (Source: Uniprot.org, NCBI)
Gene Name
AKAP4
Full Name
A-kinase anchor protein 4
Weight
94.477kDa
Superfamily
AKAP110 family
Alternative Names
A kinase (PRKA) anchor protein 4; AKAP-4; AKAP82AKAP 82; A-kinase anchor protein 4; A-kinase anchor protein 82 kDa; cancer/testis antigen 99; CT99; hAKAP82Fsc1; HIFSC1; Major sperm fibrous sheath protein; p82; PRKA4; protein kinase A anchoring protein 4; Protein kinase A-anchoring protein 4; testis-specific gene HI AKAP4 AKAP 82, AKAP-4, AKAP82, CT99, FSC1, HI, PRKA4, hAKAP82, p82 A-kinase anchoring protein 4 A-kinase anchor protein 4|A kinase (PRKA) anchor protein 4|A-kinase anchor protein 82 kDa|cancer/testis antigen 99|epididymis secretory sperm binding protein|major sperm fibrous sheath protein|protein kinase A anchoring protein 4|testis-specific gene HI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKAP4, check out the AKAP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKAP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-A-kinase anchor protein 4 AKAP4 Antibody (A07362)
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No publications found for A07362
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