Product Info Summary
SKU: | A00215-2 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-a-Synuclein (Ab-133) SNCA Antibody
View all alpha-Synuclein Antibodies
SKU/Catalog Number
A00215-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-a-Synuclein (Ab-133) SNCA Antibody (Catalog # A00215-2). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-a-Synuclein (Ab-133) SNCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00215-2)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 131~135 (E-G-Y-Q-D) derived from Human a-Synuclein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00215-2 is reactive to SNCA in Human, Mouse, Rat
Applications
A00215-2 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
14.46kDa
Background of alpha-Synuclein
May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Kozikowski AP, et al. (2006)ChemMedChem. 1(2):256-66.
Jakowec MW,et al.(2001)Dev Neurosci.23(2):91-9.
El-Agnaf OM,et al.(1998)FEBS Lett. 440(1-2):67-70.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SNCA using anti-SNCA antibody (A00215-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNCA antigen affinity purified polyclonal antibody (Catalog # A00215-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SNCA.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using a-Synuclein(Ab-133) Antibody #A00215-2.
Protein Target Info & Infographic
Gene/Protein Information For SNCA (Source: Uniprot.org, NCBI)
Gene Name
SNCA
Full Name
Alpha-synuclein
Weight
14.46kDa
Superfamily
synuclein family
Alternative Names
alpha-Synuclein; Lewy body) 4; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; non-A4 component of amyloid; PARK1; PARK4; SNCA; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha SNCA NACP, PARK1, PARK4, PD1 synuclein alpha alpha-synuclein|I+/--synuclein|non A-beta component of AD amyloid|synuclein alpha-140|synuclein, alpha (non A4 component of amyloid precursor)|truncated alpha synuclein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNCA, check out the SNCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-a-Synuclein (Ab-133) SNCA Antibody (A00215-2)
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No publications found for A00215-2
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