Product Info Summary
SKU: | A08490-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-ABCA8 Antibody
SKU/Catalog Number
A08490-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ABCA8 Antibody catalog # A08490-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCA8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08490-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human ABCA8. at AA range: 1120-1200
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08490-1 is reactive to ABCA8 in Human, Mouse
Applications
A08490-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
179.245kDa
Background of ABCA8
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. PARC is a cullin family member that functions as a cytoplasmic anchor protein in p53-associated protein complexes. PARC regulates the subcellular localization of p53 and subsequent function. PARC forms a complex with p53 in the cytoplasm of unstressed cells and interacts with UBCH7 and UBCH8. PARC shows a cytoplasmic localization and is ubiquitously expressed in all tissues with highest expression in testis brain and kidney.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HuvEc cells using ABCA8 Polyclonal Antibody
Click image to see more details
Western blot analysis of various cells using ABCA8 Polyclonal Antibody
Protein Target Info & Infographic
Gene/Protein Information For ABCA8 (Source: Uniprot.org, NCBI)
Gene Name
ABCA8
Full Name
ATP-binding cassette sub-family A member 8
Weight
179.245kDa
Superfamily
ABC transporter superfamily
Alternative Names
ATP-binding cassette sub-family A member 8; ATP-binding cassette, sub-family A (ABC1), member 8; EC 3.6.3; EC 3.6.3.41; KIAA0822MGC163152 ABCA8 ATP binding cassette subfamily A member 8 ABC-type organic anion transporter ABCA8|ATP-binding cassette, sub-family A (ABC1), member 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCA8, check out the ABCA8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCA8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCA8 Antibody (A08490-1)
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No publications found for A08490-1
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-ABCA8 Antibody
Question
I see that the anti-ABCA8 antibody A08490-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-09
Question
Is this A08490-1 anti-ABCA8 antibody reactive to the isotypes of ABCA8?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
The immunogen of A08490-1 anti-ABCA8 antibody is Synthesized peptide derived from the Internal region of human ABCA8. at AA rangle: 1120-1200. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-23
Question
We are currently using anti-ABCA8 antibody A08490-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
The anti-ABCA8 antibody (A08490-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-13