Product Info Summary
SKU: | PA1868 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ABCG1 Antibody
SKU/Catalog Number
PA1868
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABCG1 Antibody catalog # PA1868. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1868)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ABCG1, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1868 is reactive to ABCG1 in Human, Mouse, Rat
Applications
PA1868 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
75.592kDa
Background of ABCG1
ABCG1 (Atp-binding cassette, subfamily g, member 1) also known as ABC8, ABC TRANSPORTER 8 or WHITE, DROSOPHILA, HOMOLOG OF, is a protein that in humans is encoded by the ABCG1 gene. ABCG1 is a regulator of macrophage cholesterol and phospholipid transport. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters.The ABCG1 gene is mapped on 21q22.3. This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. ABCG1 is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Inhibition of ABCG1 protein expression using an antisense strategy resulted in reduced HDL3-dependent efflux of cholesterol and choline-phospholipids. In macrophages, silencing of GPS2 by RNA interference reduced ABCG1 expression and diminished ABCG1-mediated cholesterol efflux.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-ABCG1 antibody, PA1868, Western blotting
All lanes: Anti ABCG1 (PA1868) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
Protein Target Info & Infographic
Gene/Protein Information For ABCG1 (Source: Uniprot.org, NCBI)
Gene Name
ABCG1
Full Name
ATP-binding cassette sub-family G member 1
Weight
75.592kDa
Superfamily
ABC transporter superfamily
Alternative Names
ABC transporter 8; ABC8; ABC8ATP-binding cassette sub-family G member 1; ABCG1; ATP-binding cassette transporter 8; ATP-binding cassette, sub-family G (WHITE), member 1; homolog of Drosophila white; MGC34313; white protein homolog (ATP-binding cassette transporter 8), 10ATP-binding cassette transporter member 1 of subfamily G; White protein homolog; WHITE1; WHT1 ABCG1 ABC8, WHITE1 ATP binding cassette subfamily G member 1 ATP-binding cassette sub-family G member 1|ABC transporter 8|ATP-binding cassette transporter 8|ATP-binding cassette transporter member 1 of subfamily G|ATP-binding cassette, sub-family G (WHITE), member 1|homolog of Drosophila white|white protein homolog (ATP-binding cassette transporter 8)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCG1, check out the ABCG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCG1 Antibody (PA1868)
Hello CJ!
PA1868 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Overexpression of C35 in breast carcinomas is associated with tumor progression and lymphnode metastasis
Yan N, Ding T, Dong J, Li Y, Wu M. Lipids Health Dis. 2011 Mar 21;10:46. Doi: 10.1186/1476-511X-10-46. Sphingomyelin Synthase Overexpression Increases Cholesterol Accumulation And Decreases Cholesterol Secretion In Liver Cells.
Shang W, Yu X, Wang H, Chen T, Fang Y, Yang X, Zhou P, Nie F, Zhou Q, Zhou J. Mol Med Rep. 2015 Jan;11(1):503-8. Doi: 10.3892/Mmr.2014.2731. Epub 2014 Oct 21. Fibroblast Growth Factor 21 Enhances Cholesterol Efflux In Thp-1 Macrophage-Derived Foam...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-ABCG1 Antibody
Question
I see that the anti-ABCG1 antibody PA1868 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-25
Question
We are currently using anti-ABCG1 antibody PA1868 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The anti-ABCG1 antibody (PA1868) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-26
Question
Does anti-ABCG1 antibody PA1868 work on pig WB with retina?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
Our lab technicians have not tested anti-ABCG1 antibody PA1868 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ABCG1 antibody PA1868 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig retina in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-ABCG1 antibody PA1868. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
Question
See below the WB image, lot number and protocol we used for brain using anti-ABCG1 antibody PA1868. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-19
Question
Would anti-ABCG1 antibody PA1868 work for WB with brain?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
According to the expression profile of brain, ABCG1 is highly expressed in brain. So, it is likely that anti-ABCG1 antibody PA1868 will work for WB with brain.
Boster Scientific Support
Answered: 2018-11-22