Product Info Summary
SKU: | A01227-1 |
---|---|
Size: | 80 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-ABCG1 Antibody (Center)
SKU/Catalog Number
A01227-1
Size
80 µl
Description
Boster Bio Anti-ABCG1 Antibody (Center) (Catalog # A01227-1). Tested in Flow Cytometry, IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ABCG1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01227-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ABCG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 359-387 amino acids from the Central region of human ABCG1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01227-1 is reactive to ABCG1 in Human
Applications
A01227-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
75.592kDa
Background of ABCG1
ABCG1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Anti-ABCG1 Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 76 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ABCG1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Click image to see more details
ABCG1 Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For ABCG1 (Source: Uniprot.org, NCBI)
Gene Name
ABCG1
Full Name
ATP-binding cassette sub-family G member 1
Weight
75.592kDa
Superfamily
ABC transporter superfamily
Alternative Names
ABC transporter 8; ABC8; ABC8ATP-binding cassette sub-family G member 1; ABCG1; ATP-binding cassette transporter 8; ATP-binding cassette, sub-family G (WHITE), member 1; homolog of Drosophila white; MGC34313; white protein homolog (ATP-binding cassette transporter 8), 10ATP-binding cassette transporter member 1 of subfamily G; White protein homolog; WHITE1; WHT1 ABCG1 ABC8, WHITE1 ATP binding cassette subfamily G member 1 ATP-binding cassette sub-family G member 1|ABC transporter 8|ATP-binding cassette transporter 8|ATP-binding cassette transporter member 1 of subfamily G|ATP-binding cassette, sub-family G (WHITE), member 1|homolog of Drosophila white|white protein homolog (ATP-binding cassette transporter 8)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCG1, check out the ABCG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCG1 Antibody (Center) (A01227-1)
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No publications found for A01227-1
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