Anti-ABCG5 Antibody

Abcg5 antibody

Boster Bio Anti-ABCG5 Antibody catalog # PA1871. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1871
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IHC, ICC, WB

Product Name

Anti-ABCG5 Antibody

View all Abcg5 Antibodies

SKU/Catalog Number

PA1871

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ABCG5 Antibody catalog # PA1871. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ABCG5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1871)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1871 is reactive to Abcg5 in Human

Applications

PA1871 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

73 kDa

Calculated molecular weight

73.244kDa

Background of Abcg5

ABCG5 (Atp-binding cassette, subfamily g, member 5) also known as STEROLIN 1, is a protein that in humans is encoded by the ABCG5 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. The ABCG5 gene contains 13 exons and spans about 28 kb.The ABCG5 gene is mapped on 2p21. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. Small (2003) reviewed the role of ABC transporters in secretion of cholesterol from liver into bile, particularly the role of ABCG5/ABCG8.The ABCG5 and ABCG8 genes are an example of closely neighboring genes in a head-to-head orientation that, when mutated, cause the same phenotype.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For Abcg5 (Source: Uniprot.org, NCBI)

Gene Name

Abcg5

Full Name

ATP-binding cassette sub-family G member 5

Weight

73.244kDa

Superfamily

ABC transporter superfamily

Alternative Names

ATP-binding cassette sub-family G member 5; ATP-binding cassette, sub-family G (WHITE), member 5; ATP-binding cassette, subfamily G, member 5; sterolin 1; sterolin-1; STSL Abcg5|Ster, cm, cmp, sterolin-1, tr, trac|ATP binding cassette subfamily G member 5|ATP-binding cassette sub-family G member 5|ATP-binding cassette, sub-family G (WHITE), member 5|sterolin 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Abcg5, check out the Abcg5 Infographic

Abcg5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Abcg5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-ABCG5 Antibody

Question

Is this PA1871 anti-ABCG5 antibody reactive to the isotypes of ABCG5?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

The immunogen of PA1871 anti-ABCG5 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human ABCG5(116-131aa NGRALRREQFQDCFSY). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-16

Question

Do you have a BSA free version of anti-ABCG5 antibody PA1871 available?

Z. Mangal

Verified customer

Asked: 2019-08-08

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ABCG5 antibody PA1871 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-08

Question

Would anti-ABCG5 antibody PA1871 work on canine Flow Cytometry with liver?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

Our lab technicians have not tested anti-ABCG5 antibody PA1871 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ABCG5 antibody PA1871 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-04

Question

We need to test anti-ABCG5 antibody PA1871 on human liver for research purposes, then I may be interested in using anti-ABCG5 antibody PA1871 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Kulkarni

Verified customer

Asked: 2019-05-27

Answer

The products we sell, including anti-ABCG5 antibody PA1871, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-27

Question

Is a blocking peptide available for product anti-ABCG5 antibody (PA1871)?

Verified Customer

Verified customer

Asked: 2018-08-08

Answer

We do provide the blocking peptide for product anti-ABCG5 antibody (PA1871). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-08-08

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ABCG5 antibody PA1871. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-05-17

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-17

Question

I have a question about product PA1871, anti-ABCG5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

W. Taylor

Verified customer

Asked: 2017-05-10

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1871 anti-ABCG5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-05-10

Size

Conjugation

Total: $370

SKU:PA1871

In stock, 3+ left.

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PA1871
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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