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Product Info Summary
| SKU: | M03209 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-ABHD5 Monoclonal Antibody
SKU/Catalog Number
M03209
BM5774 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ABHD5 Monoclonal Antibody catalog # M03209. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABHD5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03209)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFHE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ABHD5 Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.
Reactive Species
M03209 is reactive to ABHD5 in Human
Observed Molecular Weight
45 kDa
Calculated molecular weight
39.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03209 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:100
Positive Control
WB: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of ABHD5 expression in HepG2 cell lysate.
Specific Publications For Anti-ABHD5 Monoclonal Antibody (M03209)
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Customer Q&As
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5 Customer Q&As for Anti-ABHD5 Monoclonal Antibody
Question
I was wanting to use your anti-ABHD5 Monoclonal antibody for WB for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
You can see on the product datasheet, M03209 anti-ABHD5 Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-15
Question
Is this M03209 anti-ABHD5 Monoclonal antibody reactive to the isotypes of ABHD5?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The immunogen of M03209 anti-ABHD5 Monoclonal antibody is A synthesized peptide derived from human ABHD5 Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-25
Question
Would M03209 anti-ABHD5 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
It shows on the product datasheet, M03209 anti-ABHD5 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-07
Question
I have attached the WB image, lot number and protocol we used for blood using anti-ABHD5 Monoclonal antibody M03209. Please let me know if you require anything else.
K. Jones
Verified customer
Asked: 2015-01-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-05
Question
Does anti-ABHD5 Monoclonal antibody M03209 work on goat WB with blood?
N. Bhatt
Verified customer
Asked: 2014-08-01
Answer
Our lab technicians have not tested anti-ABHD5 Monoclonal antibody M03209 on goat. You can run a BLAST between goat and the immunogen sequence of anti-ABHD5 Monoclonal antibody M03209 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-01
