Anti-ABIN3 TNIP3 Antibody

Product Name

Anti-ABIN3 TNIP3 Antibody

View all TNIP3 Antibodies

SKU/Catalog Number

A10336

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ABIN3 TNIP3 Antibody (Catalog # A10336). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.

Storage & Handling

ABIN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-ABIN3 TNIP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10336)

Host

Rabbit

Contents

ABIN3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

ABIN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ABIN3. The immunogen is located within the last 50 amino acids of ABIN3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of UPK1B are known to exist; this antibody will recognize both isoforms. UPK1B antibody is predicted to not cross-react with other tetraspanin protein family members.

Reactive Species

A10336 is reactive to TNIP3 in Human, Mouse

Applications

A10336 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

38.943kDa

Background of TNIP3

The nuclear factor NF-κB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-κB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-κB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-κB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-κB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-κB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ABIN3 antibody can be used for detection of ABIN3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

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Immunohistochemistry of ABIN3 in mouse spleen tissue with ABIN3 antibody at 5 μg/mL.

Gene/Protein Information For TNIP3 (Source: Uniprot.org, NCBI)

Alternative Names

A20; A20-binding inhibitor of NF-kappa-B activation 3; ABIN3; ABIN-3ABIN-3 beta; FLJ21162; LINDListeria induced; Listeria-induced gene protein; TNFAIP3 interacting protein 3; TNFAIP3-interacting protein 3 beta; TNFAIP3-interacting protein 3; TNIP3 beta TNIP3 ABIN-3, LIND TNFAIP3 interacting protein 3 TNFAIP3-interacting protein 3|A20-binding inhibitor of NF-kappa-B activation 3|ABIN-3 beta|Listeria induced|TNFAIP3-interacting protein 3 beta|TNIP3 beta|listeria-induced gene protein

For more info on TNIP3, check out the TNIP3 Infographic

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In this infographic, you will see the following information for TNIP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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