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Product Info Summary
| SKU: | M00756 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ACE2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00756
BM4624 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ACE2 Rabbit Monoclonal Antibody catalog # M00756. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACE2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00756)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GEE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ACE2
Reactive Species
M00756 is reactive to ACE2 in Human, Mouse, Rat
Observed Molecular Weight
92 kDa
Calculated molecular weight
92.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00756 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:20-100
ICC/IF 1:20-100
IP 1:20
Positive Control
IHC: Rat liver tissue, Rat stomach tissue, Human lung large cell cancer tissue, Human liver cancer tissue, Human squamous cell carcinoma tissue, human kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of ACE2 expression in Human kidney lysate.
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Immunohistochemical analysis of paraffin-embedded Rat liver, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver cancer, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma , using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human kidney, using ACE2 Antibody.
Specific Publications For Anti-ACE2 Rabbit Monoclonal Antibody (M00756)
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Customer Q&As
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5 Customer Q&As for Anti-ACE2 Rabbit Monoclonal Antibody
Question
Would M00756 anti-ACE2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
It shows on the product datasheet, M00756 anti-ACE2 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
We are currently using anti-ACE2 Rabbit Monoclonal antibody M00756 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The anti-ACE2 Rabbit Monoclonal antibody (M00756) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-14
Question
Do you have a BSA free version of anti-ACE2 Rabbit Monoclonal antibody M00756 available?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ACE2 Rabbit Monoclonal antibody M00756 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-31
Question
Would anti-ACE2 Rabbit Monoclonal antibody M00756 work on canine IF with jejunal mucosa?
L. Thomas
Verified customer
Asked: 2016-10-21
Answer
Our lab technicians have not validated anti-ACE2 Rabbit Monoclonal antibody M00756 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ACE2 Rabbit Monoclonal antibody M00756 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine jejunal mucosa in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-21
Question
Will anti-ACE2 Rabbit Monoclonal antibody M00756 work for WB with bile?
W. Krishna
Verified customer
Asked: 2016-04-18
Answer
According to the expression profile of bile, ACE2 is highly expressed in bile. So, it is likely that anti-ACE2 Rabbit Monoclonal antibody M00756 will work for WB with bile.
Boster Scientific Support
Answered: 2016-04-18
