Anti-Acid ceramidase ASAH1 Antibody

Product Name

Anti-Acid ceramidase ASAH1 Antibody

View all ASAH1 Antibodies

SKU/Catalog Number

A02055

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Acid ceramidase ASAH1 Antibody (Catalog # A02055). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ASAH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Acid ceramidase ASAH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02055)

Host

Rabbit

Contents

ASAH1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ASAH1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human ASAH1. The immunogen is located within amino acids 240 - 290 of ASAH1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Multiple isoforms of ASAH1 are known to exist.

Reactive Species

A02055 is reactive to ASAH1 in Human, Mouse, Rat

Applications

A02055 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

44.66kDa

Background of ASAH1

Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH1 is an acid ceramidase and key regulator of ceramide metabolism. Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulminary insufficiency, while a complete knockout of its expression is lethal in mice. Recent studies have shown elevated levels of ASAH1 in Alzheimer's disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide. Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ASAH1 antibody can be used for detection of ASAH1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of ASAH1 in human heart tissue lysate with ASAH1 antibody at 1 μg/mL.

Click image to see more details

Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5 μg/mL.

Click image to see more details

Immunofluorescence of ASAH1 in Human Heart cells with ASAH1 antibody at 20 μg/mL.

Click image to see more details

Immunohistochemistry of ASAH1 in rat heart tissue with ASAH1 antibody at 5 μg/mL.

Click image to see more details

Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 μg/mL.

Red: ASAH1 Antibody (A02055)
Blue: DAPI staining

Gene/Protein Information For ASAH1 (Source: Uniprot.org, NCBI)

Alternative Names

ACDase; ACEC 3.5.1.23; Acid CDase; acid ceramidase; Acylsphingosine deacylase; ASAH; FLJ21558; FLJ22079; N-acylsphingosine amidohydrolase (acid ceramidase) 1; N-acylsphingosine amidohydrolase; PHP; PHP32N-acylsphingosine amidohydrolase (acid ceramidase); Putative 32 kDa heart protein ASAH1 AC, ACDase, ASAH, PHP, PHP32, SMAPME N-acylsphingosine amidohydrolase 1 acid ceramidase|N-acylethanolamine hydrolase ASAH1|N-acylsphingosine amidohydrolase (acid ceramidase) 1|acid CDase|acylsphingosine deacylase|putative 32 kDa heart protein

For more info on ASAH1, check out the ASAH1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ASAH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the ASAH1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis