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Product Info Summary
| SKU: | M02781-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ACO1 Antibody (N-Term)
SKU/Catalog Number
M02781-1
Size
50 µl
Description
Boster Bio Anti-ACO1 Antibody (N-Term) (Catalog # M02781-1). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ACO1 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02781-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ACO1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 124-155 amino acids from human ACO1.
Reactive Species
M02781-1 is reactive to ACO1 in Human, Mouse, Rat
Calculated molecular weight
98.4 kDa
Background of ACO1
Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02781-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-ACO1 Antibody (N-Term) at 1:2000 dilution
Lane 1: U-2OS whole cell lysate
Lane 2: mouse kidney lysate
Lane 3: rat kidney lysate
Lane 4: human kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 98 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-ACO1 Antibody (N-Term) (M02781-1)
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