Anti-Acyl-coenzyme A thioesterase 4 ACOT4 Antibody

ACOT4 antibody

Boster Bio Anti-Acyl-coenzyme A thioesterase 4 ACOT4 Antibody (Catalog # A11674). Tested in WB, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A11674
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, WB

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Product Name

Anti-Acyl-coenzyme A thioesterase 4 ACOT4 Antibody

View all ACOT4 Antibodies

SKU/Catalog Number

A11674

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Acyl-coenzyme A thioesterase 4 ACOT4 Antibody (Catalog # A11674). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Acyl-coenzyme A thioesterase 4 ACOT4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11674)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from internal of human ACOT4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11674 is reactive to ACOT4 in Human

Applications

A11674 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

46.327kDa

Background of ACOT4

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH By similarity. Succinyl-CoA thioesterase that also hydrolyzes long chain saturated and unsaturated monocarboxylic acyl-CoAs.

Ota T., Nat. Genet. 36:40-45(2004).
Li W.B., Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For ACOT4 (Source: Uniprot.org, NCBI)

Gene Name

ACOT4

Full Name

Peroxisomal succinyl-coenzyme A thioesterase

Weight

46.327kDa

Superfamily

C/M/P thioester hydrolase family

Alternative Names

acyl-CoA thioesterase 4EC 3.1.2.2; acyl-coenzyme A thioesterase 4; Peroxisomal acyl coenzyme A thioester hydrolase Ib; peroxisomal acyl-CoA thioesterase 2B; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-2b; PTE2BPTE1B; PTEIB; PTE-IbFLJ31235 ACOT4 PTE-Ib, PTE1B, PTE2B acyl-CoA thioesterase 4 peroxisomal succinyl-coenzyme A thioesterase|PTE-2b|acyl-coenzyme A thioesterase 4|peroxisomal acyl coenzyme A thioester hydrolase Ib|peroxisomal acyl-CoA thioesterase 2B|peroxisomal long-chain acyl-CoA thioesterase Ib

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACOT4, check out the ACOT4 Infographic

ACOT4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACOT4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Acyl-coenzyme A thioesterase 4 ACOT4 Antibody

Question

Is this A11674 anti-ACOT4 antibody reactive to the isotypes of ACOT4?

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

The immunogen of A11674 anti-ACOT4 antibody is Synthesized peptide derived from internal of human ACOT4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-24

Question

We are currently using anti-ACOT4 antibody A11674 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-19

Answer

The anti-ACOT4 antibody (A11674) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-19

Question

My question regarding product A11674, anti-ACOT4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Anderson

Verified customer

Asked: 2016-12-06

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11674 anti-ACOT4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-12-06

Question

Would anti-ACOT4 antibody A11674 work on zebrafish WB with colon urinary bladder?

V. Roberts

Verified customer

Asked: 2013-11-04

Answer

Our lab technicians have not tested anti-ACOT4 antibody A11674 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ACOT4 antibody A11674 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish colon urinary bladder in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-11-04

Size

Total: $360

SKU:A11674

Backordered.

Lead time for this item is typically 2-3 weeks

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A11674
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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