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Product Info Summary
| SKU: | A06554-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ACP2 Antibody Picoband®
SKU/Catalog Number
A06554-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ACP2 Antibody Picoband® catalog # A06554-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ACP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06554-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ACP2, which shares 100% and 96.4% amino acid (aa) sequence identity with mouse and rat ACP2, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06554-1 is reactive to ACP2 in Human, Mouse, Rat
Observed Molecular Weight
76 kDa
Calculated molecular weight
48.3 kDa
Background of ACP2
Lysosomal acid phosphatase is an enzyme that in humans is encoded by the ACP2 gene. The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene in mice showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Alternatively spliced transcript variants have been found for this gene. A C-terminally extended isoform is also predicted to be produced by the use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06554-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Positive Control
WB: human T-47D whole cell, human Hela whole cell, human U251 whole cell, human RT4 whole cell, rat liver tissue, rat pancreas tissue, mouse liver tissue, mouse pancreas tissue
Validation Images & Assay Conditions
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Western blot analysis of ACP2 using anti-ACP2 antibody (A06554-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T-47D whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat pancreas tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACP2 antigen affinity purified polyclonal antibody (Catalog # A06554-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACP2 at approximately 76 kDa. The expected band size for ACP2 is at 76 kDa.
Specific Publications For Anti-ACP2 Antibody Picoband® (A06554-1)
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