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ACTA2 antibody (M01072-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 100 Publications

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Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody .

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Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

Name: Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: M01072-1
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

ACTA2 antibody

100 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

ACTA2 antibody was positively detected by WB in:

human A549 whole cell, human Hela whole cell, human 293T whole cell, human HepG2 whole cell, human Caco-2 whole cell, human SH-SY5Y whole cell, human U251 whole cell, human MCF-7 whole cell, rat heart tissue, rat lung tissue, rat brain tissue, mouse heart tissue, mouse lung tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ACTA2 antibody was positively detected by IHC in:

human colon tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

ACTA2 antibody was positively detected by ICC/IF in:

A431 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
View all ACTA2 Antibodies

Form

Liquid

Uniprot

P62736

Gene ID

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

EO-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human alpha smooth muscle Actin

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

42 kDa

Calculated Molecular Weight

42.0 kDa

Cite This Product

Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01072-1)

Protocols

Western Blot protocol for ACTA2 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for ACTA2 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ACTA2 antibody

Review Protocol

Publications

SPI1 promotes endothelial cell ferroptosis and intracranial aneurysm progression via EP300-mediated FOSL2 acetylation

Published: 2026-06-17

Journal: JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM

Application: IF

Impact Factor: 4.70

Species: Mouse

PMID: 42310495

SKU	M01072-1
Application	Western Blot
Sample	mouse cardiac tissue
Sample Processing Description	Mouse cardiac tissues were collected from 3-, 6-, 12-, and 18-month-old mice, and total protein was extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h at RT
Detection	Substrate: ECL substrate Imaging system: ChemiDoc MP
Results Summary	This experiment aimed to investigate the changes in α-SMA expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, and aged stages). The results show that α-SMA levels are generally similar across different ages, with a slight but not significant increase as age progresses.

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Anti-alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody

ACTA2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

SPI1 promotes endothelial cell ferroptosis and intracranial aneurysm progression via EP300-mediated FOSL2 acetylation

Astaxanthin Alleviates Bleomycin-Induced Pulmonary Fibrosis in Mice by Suppressing the Fibroblast-to-Myofibroblast Transition and Collagen Deposition

Single-Cell Transcriptomic Profiling of Human Blood Vessel Organoids Exposed to 6-Chloronicotinic Acid

Miltefosine attenuates silicosis involving inhibiting the Akt/mTOR signaling pathway

NUDT21 Downregulation Promotes Liver Fibrosis by Activating the PIM2-Mediated CEBPB/SLC2A1 Glycolytic Axis in Hepatic Stellate Cells

Rats develop more robust and persistent peritoneal adhesion phenotypes than mice: a multimodal comparative study

Mechanisms of Mulberry Leaf Extracts Ameliorating Inflammation and Fibrosis in Non-Alcoholic Fatty Liver Disease Mice via NF-κB and TGFβ1/Smad3 Pathways

Rhodiosin promotes cerebral angiogenesis in mice with ischemic stroke via PI3K/Akt pathway activation

Capsaicin Receptor TRPV1 Delays Aortic Aging in Atherosclerotic Mice by Inhibiting the ISG15-p53 Pathway

MDK promotes the mast cell activation and pancreatic fibrosis in mice with chronic pancreatitis via MDK-NCL signaling pathway

Nephropathy 1 Formula alleviates kidney injury by ameliorating mitochondrial dysfunction and pyroptosis in diabetic nephropathy through the TMAO-mROS-NLRP3 axis

Freeze-Drying Tumor Tissues Derived Bio-Patches With Hair Melanin Nanoparticles Integration for Wound Healing

Bifunctional Artificial Enzymes-Loaded Microgels With LOX- and CAT-Like Activities for Metabolic Reprogramming and Scarless Wound Repair

A chitosan-based thermosensitive hydrogel incorporating propranolol nanoparticles to promote early osseointegration and peri-implant soft-tissue healing

Complement protein C1q induces endothelial apoptosis through C1qBP regulation of mitochondrial function in lung endothelial cells in systemic sclerosis

Carrier-free dual-drug nanoplatforms cooperatively remodel macrophage and VSMC fate specifications for treating abdominal aortic aneurysm

Phosphate binders suppress glutaminase activity for treatment of cirrhosis and hepatic encephalopathy murine models

Taraxasterol Ameliorates Pulmonary Fibrosis by Regulating PPP2R1B Expression

DNMT3A-dependent suppression of HIF-1α alleviates myopia by reducing scleral ferroptosis and fibroblast extracellular matrix remodeling

Combining transcriptomics with network pharmacology to explore the mechanism of Yiqi Huoxue decoction against liver fibrosis

MYST1-Mediated Lysine Acetylation Stabilizes KLF4 to Promote Intimal Hyperplasia

Ustiloxins induce kidney injury via the TLR2/MAPK/NF-κB pathway

4EBP1 phosphorylation mediates TGF-β1–induced hepatic fibrosis and upregulates HIF-1α expression

Muscone attenuates myocardial infarction by regulating ferroptosis of cardiomyocytes through Nrf2/system Xc-/GPX4 signaling pathway

Prkcq in ILC2 cells-enhanced the amounts of tissue-resident macrophages promotes pancreatic fibrosis in chronic pancreatitis

Quercetagetin alleviates liver fibrosis in non-alcoholic fatty liver disease by promoting ferroptosis of hepatic stellate cells through GPX4 ubiquitination

Effect of Porphyromonas gingivalis outer membrane vesicles on renal tubule epithelial–mesenchymal transition

Yijing Decoction improves premature ovarian failure in rats by activating VEGF/VEGFR-2/FAK pathway

HIF-1α/LTBP2 axis activate HSCs to promote liver fibrosis by interacting with LOXL1 via the ERK pathway

Intermedin protects peritubular capillaries by inhibiting eNOS uncoupling through AMPK/GTPCH-I/BH4 pathway and alleviate CKD following AKI

Astragalus mongholicusbunge and Angelica sinensis botanical drug decoction mitigates lung inflammation through NOX4/TGF-β1/SMAD3 signaling

Exosomal SphK1 from colorectal cancer cells promotes cancer cell migration and activates hepatic stellate cells

Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation

Degraded products generated by iron stent inhibit the vascular smooth muscle cell proliferation by downregulating AP-1

PDPN+ cancer-associated fibroblasts enhance gastric cancer angiogenesis via AKT/NF-κB activation and the CCL2-ACKR1 axis

3D bioprinting of a dermal scaffold for full-thickness skin tissue regeneration

Zhishi Xiebai Guizhi Decoction modulates hypoxia and lipid toxicity to alleviate pulmonary vascular remodeling of pulmonary hypertension in rats

Pulling the Rug Out from Under: Biomechanical Microenvironment Remodeling for Induction of Hepatic Stellate Cell Quiescence

Lactucin reverses liver fibrosis by inhibiting TGF-β1/STAT3 signaling pathway and regulating short-chain fatty acids metabolism

MFN2-dependent mitochondrial dysfunction contributes to Relm-β-induced pulmonary arterial hypertension via USP18/Twist1/miR-214 pathway

Microbial-derived Urolithin A targets GLS1 to inhibit glutaminolysis and attenuate cirrhotic portal hypertension

Heteryunine A, an amidated tryptophan-catechin-spiroketal hybrid with antifibrotic activity from Heterosmilax yunnanensis

High Hydrostatic Pressure Exacerbates Bladder Fibrosis through Activating Piezo1

Harmine inhibits pulmonary fibrosis through regulating DNA damage repair-related genes and activation of TP53-Gadd45α pathway

Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model

Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm

Dysregulated VEGF/VEGFR-2 Signaling and Plexogenic Lesions in the Embryonic Lungs of Chickens Predisposed to Pulmonary Arterial Hypertension

Loss of ZNF451 mediates fibroblast activation and promotes lung fibrosis

Hydrogen sulfide regulates macrophage polarization and necroptosis to accelerate diabetic skin wound healing

Innovative approach: utilizing silver nanoparticles sheet for improved rabbit cecal anastomosis healing

RNA-seq combined network pharmacology reveals that Fu-Gan-Wan (FGW) inhibits liver fibrosis via NF-κB/CCL2/CCR2 and lipid peroxidation via Nrf2/HMOX1 signaling pathway

The pathogenic role of succinate-SUCNR1: a critical function that induces renal fibrosis via M2 macrophage

Efficient intervention for pulmonary fibrosis via mitochondrial transfer promoted by mitochondrial biogenesis

Exploration of Perturbed Liver Fibrosis-Related Factors and Collagen Type I in Animal Model of Non-Alcoholic Fatty Liver Disease

Remodeling of the liver fibrosis microenvironment based on nilotinib-loaded multicatalytic nanozymes with boosted antifibrogenic activity

The suppression of hyperlipid diet-induced ferroptosis of vascular smooth muscle cells protests against atherosclerosis independent of p53/SCL7A11/GPX4 axis

Murine Cytomegalovirus Infection Induced miR-1929-3p Down-Regulation Promotes the Proliferation and Apoptosis of Vascular Smooth Muscle Cells in Mice by Targeting Endothelin A Receptor and Downstream NLRP3 Activation Pathway

Poly(ferulic acid)-hybrid nanofibers for reducing thrombosis and restraining intimal hyperplasia in vascular tissue engineering

FADD phosphorylation contributes to development of renal fibrosis by accelerating epithelial-mesenchymal transition

Capsaicin Ameliorates High-Fat Diet-Induced Atherosclerosis in ApoE−/− Mice via Remodeling Gut Microbiota

Tibetan Medicine Shi-Wei-Gan-Ning-San Alleviates Carbon Tetrachloride-Induced Chronic Liver Injury by Inhibiting TGF-β1 in Wistar Rats

Inhibition of protein arginine methyltransferase 1 alleviates liver fibrosis by attenuating the activation of hepatic stellate cells in mice

PBAT/gelatin hybrid nanofibers based on post-double network bond processing as a promising vascular substitute

Identification of microRNAs related with neural germ layer lineage-specific progenitors during reprogramming

TRAF6 Suppresses the Development of Pulmonary Fibrosis by Attenuating the Activation of Fibroblasts

Endothelial cell ferroptosis mediates monocrotaline-induced pulmonary hypertension in rats by modulating NLRP3 inflammasome activation

Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways