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Product Info Summary
| SKU: | M01072-4 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253
View all alpha-Smooth Muscle Actin Antibodies
SKU/Catalog Number
M01072-4
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253 (Catalog # M01072-4). Tested in IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01072-4)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM253
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to the N-terminus of human alpha smooth muscle Actin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human and mouse alpha smooth muscle Actin (alpha-Actin-2). This antibody may also react to bovine or rat alpha smooth muscle Actin, as predicted by immunogen homology
Reactive Species
M01072-4 is reactive to ACTA2 in Human, Mouse
Applications
M01072-4 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
42.009kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:2500 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of mouse heart tissue lysate, using Anti-alpha smooth muscle Actin RM253 at a 1:1000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human heart tissue sections using Anti-alpha smooth muscle Actin RM253 at a 1:2500 dilution.
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Figure 3. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue sections using Anti-alpha smooth muscle Actin RM253 at a 1:2500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ACTA2 (Source: Uniprot.org, NCBI)
Gene Name
ACTA2
Full Name
Actin, aortic smooth muscle
Weight
42.009kDa
Superfamily
actin family
Alternative Names
1a4 sma; 1a4 smooth muscle actin; AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha sma 1a4; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; sma 1a4; SMA; smooth muscle actin ACTA2 ACTSA actin alpha 2, smooth muscle actin, aortic smooth muscle|actin, alpha 2, smooth muscle, aorta|alpha-cardiac actin|cell growth-inhibiting gene 46 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTA2, check out the ACTA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253 (M01072-4)
Hello CJ!
M01072-4 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
Species: Human
M01072-4 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
Yang J, Li J, Tan R, He X, Lin X, Zhong X, Fan J, Wang L. Protocatechualdehyde attenuates obstructive nephropathy through inhibiting lncRNA9884 induced inflammation. Phytother Res. 2020 Oct 28. doi:10.1002/ptr.6 919. Epub ahead of print. PMID: 33118280.
Species: Mouse
M01072-4 usage in article: APP:WB, SAMPLE:MOUSE KIDNEY TISSUES, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-ACTA2 Rabbit Monoclonal Antibody, Clone#RM253
Question
We are currently using anti-ACTA2 Rabbit Monoclonal antibody, Clone#RM253 M01072-4 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The anti-ACTA2 Rabbit Monoclonal antibody, Clone#RM253 (M01072-4) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-05
