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Product Info Summary
| SKU: | A05066 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Actin alpha3 ACTG2 Antibody
SKU/Catalog Number
A05066
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Actin alpha3 ACTG2 Antibody catalog # A05066. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Actin alpha3 ACTG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05066)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Actin-gamma2. AA range:1-50
Reactive Species
A05066 is reactive to ACTG2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
41.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05066 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actin-gamma2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using Actin α3 Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of COLO205 cells using Actin α3 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from COLO205 cells, using Actin-gamma2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Actin alpha3 ACTG2 Antibody (A05066)
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3 Customer Q&As for Anti-Actin alpha3 ACTG2 Antibody
Question
Does anti-Actin alpha3 antibody A05066 work on bovine WB with myometrium?
S. Mangal
Verified customer
Asked: 2020-01-20
Answer
Our lab technicians have not tested anti-Actin alpha3 antibody A05066 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Actin alpha3 antibody A05066 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine myometrium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
We are currently using anti-Actin alpha3 antibody A05066 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
The anti-Actin alpha3 antibody (A05066) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-30
Question
I have a question about product A05066, anti-Actin alpha3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05066 anti-Actin alpha3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-12
