Product Info Summary
SKU: | M02014-3 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Actin ACTA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02014-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Actin ACTA1 Rabbit Monoclonal Antibody catalog # M02014-3. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Actin ACTA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02014-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAOG-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Actin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02014-3 is reactive to ACTA1 in Human, Mouse, Rat
Applications
M02014-3 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
42.051kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Actin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ACTA1 (Source: Uniprot.org, NCBI)
Gene Name
ACTA1
Full Name
Actin, alpha skeletal muscle
Weight
42.051kDa
Superfamily
actin family
Alternative Names
ACTA; Actin; actin, alpha 1, skeletal muscle; alpha skeletal muscle actin; alpha skeletal muscle; alpha-actin-1; ASMA; CFTD; CFTDM; MPFD; NEM1; NEM2; NEM3 ACTA1 ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, SHPM actin alpha 1, skeletal muscle actin, alpha skeletal muscle|nemaline myopathy type 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTA1, check out the ACTA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Actin ACTA1 Rabbit Monoclonal Antibody (M02014-3)
Hello CJ!
M02014-3 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cheng J,Luan J,Chen P,Kuang X,Jiang P,Zhang R,Chen S,Cheng F,Gou X.Immunosuppressive receptor LILRB1 acts as a potential regulator in hepatocellular carcinoma by integrating with SHP1.Cancer Biomark.2020; 28(3):309-319.doi:10.3233/CBM-190940.PMID:32390601
Species: Human
Expression changes of ionic channels in early phase of cultured rat atrial myocytes induced by rapid pacing
The Effect of 5?-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co ?-ray
Inhibition of human La protein by RNA interference downregulates hepatitis B virus mRNA in 2.2.15 cells
Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats
Li Ys, Chen Bf, Li Xj, Zhang Wk, Tang Hb. Plos One. 2014 Sep 24;9(9):E107361. Doi: 10.1371/Journal.Pone.0107361. Ecollection 2014. Cytotoxicity Of Polyaniline Nanomaterial On Rat Celiac Macrophages In Vitro.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Actin ACTA1 Rabbit Monoclonal Antibody
Question
Will anti-Actin Rabbit Monoclonal antibody M02014-3 work for IF with skeletal muscle?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
According to the expression profile of skeletal muscle, ACTA1 is highly expressed in skeletal muscle. So, it is likely that anti-Actin Rabbit Monoclonal antibody M02014-3 will work for IF with skeletal muscle.
Boster Scientific Support
Answered: 2020-03-17
Question
I have a question about product M02014-3, anti-Actin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02014-3 anti-Actin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-02
Question
We ordered your anti-Actin Rabbit Monoclonal antibody for IF on skeletal muscle in a previous project. I am using mouse, and We are going to use the antibody for WB next. My question regards examining skeletal muscle as well as biceps brachii in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
I have checked the website and datasheets of our anti-Actin Rabbit Monoclonal antibody and it appears that M02014-3 has been tested on mouse in both IF and WB. Thus M02014-3 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-13
Question
I was wanting to use your anti-Actin Rabbit Monoclonal antibody for IF for rat skeletal muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat skeletal muscle identification?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
As indicated on the product datasheet, M02014-3 anti-Actin Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-Actin Rabbit Monoclonal antibody M02014-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
Would M02014-3 anti-Actin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
You can see on the product datasheet, M02014-3 anti-Actin Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-01
Question
We are currently using anti-Actin Rabbit Monoclonal antibody M02014-3 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
The anti-Actin Rabbit Monoclonal antibody (M02014-3) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-10
Question
My team were satisfied with the WB result of your anti-Actin Rabbit Monoclonal antibody. However we have observed positive staining in skeletal muscle cytoskeleton. using this antibody. Is that expected? Could you tell me where is ACTA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
Based on literature, skeletal muscle does express ACTA1. Generally ACTA1 expresses in cytoplasm, cytoskeleton. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334
Boster Scientific Support
Answered: 2019-06-04
Question
Is there a BSA free version of anti-Actin Rabbit Monoclonal antibody M02014-3 available?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Actin Rabbit Monoclonal antibody M02014-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-13
Question
My lab would like to test anti-Actin Rabbit Monoclonal antibody M02014-3 on rat skeletal muscle for research purposes, then I may be interested in using anti-Actin Rabbit Monoclonal antibody M02014-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
The products we sell, including anti-Actin Rabbit Monoclonal antibody M02014-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-06
Question
I see that the anti-Actin Rabbit Monoclonal antibody M02014-3 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-19
Question
Is this M02014-3 anti-Actin Rabbit Monoclonal antibody reactive to the isotypes of ACTA1?
M. Krishna
Verified customer
Asked: 2018-08-20
Answer
The immunogen of M02014-3 anti-Actin Rabbit Monoclonal antibody is A synthesized peptide derived from human Actin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-20
Question
Is a blocking peptide available for product anti-Actin Rabbit Monoclonal antibody (M02014-3)?
Verified Customer
Verified customer
Asked: 2017-11-10
Answer
We do provide the blocking peptide for product anti-Actin Rabbit Monoclonal antibody (M02014-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-10
Question
Here is the WB image, lot number and protocol we used for skeletal muscle using anti-Actin Rabbit Monoclonal antibody M02014-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-19
Question
I am interested in using your anti-Actin Rabbit Monoclonal antibody for nemaline myopathy 3 (nem3) studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
J. Williams
Verified customer
Asked: 2015-10-02
Answer
Thank you for your inquiry. This M02014-3 anti-Actin Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-10-02
Question
We have been able to see staining in rat skeletal muscle. What should we do? Is anti-Actin Rabbit Monoclonal antibody supposed to stain skeletal muscle positively?
N. Jackson
Verified customer
Asked: 2013-03-01
Answer
Based on literature skeletal muscle does express ACTA1. Based on Uniprot.org, ACTA1 is expressed in biceps brachii, skeletal muscle, among other tissues. Regarding which tissues have ACTA1 expression, here are a few articles citing expression in various tissues:
Skeletal muscle, Pubmed ID: 6190133, 15489334
Boster Scientific Support
Answered: 2013-03-01