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Product Info Summary
| SKU: | M00334-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-active Caspase-3 CASP3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00334-3
BM3937 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-active Caspase-3 CASP3 Rabbit Monoclonal Antibody catalog # M00334-3. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-active Caspase-3 CASP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-3)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human active Caspase-3
Reactive Species
M00334-3 is reactive to CASP3 in Human
Observed Molecular Weight
17 kDa
Calculated molecular weight
31.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00334-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: Jurkat cell
ICC/IF: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of active Caspase-3 expression in Jurkat cell lysate treated with Camptothecin.
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Immunofluorescent analysis of HepG2 cells, using active Caspase-3 Antibody .
Specific Publications For Anti-active Caspase-3 CASP3 Rabbit Monoclonal Antibody (M00334-3)
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5 Customer Q&As for Anti-active Caspase-3 CASP3 Rabbit Monoclonal Antibody
Question
Our team were happy with the WB result of your anti-active Caspase-3 Rabbit Monoclonal antibody. However we have observed positive staining in lymph cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
According to literature, lymph does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-06
Question
We ordered your anti-active Caspase-3 Rabbit Monoclonal antibody for ICC on lymph in the past. I am using human, and We want to use the antibody for IHC next. I was wanting to use examining lymph as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
I viewed the website and datasheets of our anti-active Caspase-3 Rabbit Monoclonal antibody and it seems that M00334-3 has been tested on human in both ICC and IHC. Thus M00334-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-31
Question
you antibody using your anti-active Caspase-3 Rabbit Monoclonal antibody for hippocampus development studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
We appreciate your inquiry. This M00334-3 anti-active Caspase-3 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-16
Question
We are currently using anti-active Caspase-3 Rabbit Monoclonal antibody M00334-3 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
The anti-active Caspase-3 Rabbit Monoclonal antibody (M00334-3) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-03
Question
We have observed staining in human lymph. Any tips? Is anti-active Caspase-3 Rabbit Monoclonal antibody supposed to stain lymph positively?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
According to literature lymph does express CASP3. According to Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-08-20
