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Product Info Summary
| SKU: | M05553 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ACTL6A Monoclonal Antibody
SKU/Catalog Number
M05553
BM5698 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ACTL6A Monoclonal Antibody catalog # M05553. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACTL6A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05553)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFCF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ACTL6A Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) . Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix.
Reactive Species
M05553 is reactive to ACTL6A in Human, Mouse, Rat
Observed Molecular Weight
47 kDa
Calculated molecular weight
47.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05553 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
Validation Images & Assay Conditions
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Western blot analysis of ACTL6A expression in Ramos cell lysate.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Specific Publications For Anti-ACTL6A Monoclonal Antibody (M05553)
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3 Customer Q&As for Anti-ACTL6A Monoclonal Antibody
Question
I was wanting to use your anti-ACTL6A Monoclonal antibody for IP for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
It shows on the product datasheet, M05553 anti-ACTL6A Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-11
Question
We are currently using anti-ACTL6A Monoclonal antibody M05553 for rat tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The anti-ACTL6A Monoclonal antibody (M05553) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
Question
Does anti-ACTL6A Monoclonal antibody M05553 work for IP with testis?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
According to the expression profile of testis, ACTL6A is highly expressed in testis. So, it is likely that anti-ACTL6A Monoclonal antibody M05553 will work for IP with testis.
Boster Scientific Support
Answered: 2017-11-15
