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Product Info Summary
| SKU: | M03673 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-ACTN2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03673
BM4907 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ACTN2 Rabbit Monoclonal Antibody catalog # M03673. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACTN2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03673)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOCB-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ACTN2
Reactive Species
M03673 is reactive to ACTN2 in Human, Mouse, Rat
Observed Molecular Weight
104 kDa
Calculated molecular weight
103.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03673 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: HepG2 cell, rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue
Validation Images & Assay Conditions
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Western blot analysis of ACTN2 expression in HepG2 cell lysate.
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Western blot analysis of ACTN2 using anti-ACTN2 antibody (M03673).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates,
Lane 4: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN2 antigen affinity purified monoclonal antibody (Catalog # M03673) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTN2 at approximately 104 kDa. The expected band size for ACTN2 is at 104 kDa.
Specific Publications For Anti-ACTN2 Rabbit Monoclonal Antibody (M03673)
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3 Customer Q&As for Anti-ACTN2 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-ACTN2 Rabbit Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
You can see on the product datasheet, M03673 anti-ACTN2 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-25
Question
Our lab want to know about to test anti-ACTN2 Rabbit Monoclonal antibody M03673 on human brain for research purposes, then I may be interested in using anti-ACTN2 Rabbit Monoclonal antibody M03673 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The products we sell, including anti-ACTN2 Rabbit Monoclonal antibody M03673, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-28
Question
Is this M03673 anti-ACTN2 Rabbit Monoclonal antibody reactive to the isotypes of ACTN2?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
The immunogen of M03673 anti-ACTN2 Rabbit Monoclonal antibody is A synthesized peptide derived from human ACTN2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-08
