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Product Info Summary
| SKU: | A04770-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ACVR2A Antibody Picoband®
SKU/Catalog Number
A04770-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-ACVR2A Antibody Picoband® catalog # A04770-2. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ACVR2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04770-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human ACVR2A recombinant protein (Position: A20-N138). Human ACVR2A shares 98.3% amino acid (aa) sequence identity with mouse and rat ACVR2A.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04770-2 is reactive to ACVR2A in Human
Observed Molecular Weight
58 kDa
Calculated molecular weight
57.8 kDa
Background of ACVR2A
Activin receptor type-2A is a protein that in humans is encoded by the ACVR2A gene. ACVR2A is an activin type 2 receptor. This gene encodes a receptor that mediates the functions of activins, which are members of the transforming growth factor-beta (TGF-beta) superfamily involved in diverse biological processes. The encoded protein is a transmembrane serine-threonine kinase receptor which mediates signaling by forming heterodimeric complexes with various combinations of type I and type II receptors and ligands in a cell-specific manner. The encoded type II receptor is primarily involved in ligand-binding and includes an extracellular ligand-binding domain, a transmembrane domain and a cytoplasmic serine-threonine kinase domain. This gene may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality. Alternative splicing results in multiple transcript variants of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04770-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human RT4 whole cell, human SH-SY5Y whole cell, human 293T whole cell
Validation Images & Assay Conditions
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Western blot analysis of ACVR2A using anti-ACVR2A antibody (A04770-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR2A antigen affinity purified polyclonal antibody (Catalog # A04770-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR2A at approximately 58 kDa. The expected band size for ACVR2A is at 58 kDa.
Specific Publications For Anti-ACVR2A Antibody Picoband® (A04770-2)
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