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Product Info Summary
| SKU: | A02264 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ADAM12 Antibody
SKU/Catalog Number
A02264
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ADAM12 Antibody catalog # A02264. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAM12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02264)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide corresponding to residues in Human ADAM12
Reactive Species
A02264 is reactive to ADAM12 in Human, Mouse, Rat
Calculated molecular weight
99.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02264 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of ADAM12 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
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Immunohistochemistry (IHC) analyzes of ADAM12 pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasmic,membrane and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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Immunohistochemistry (IHC) analyzes of ADAM12 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50,showing cytoplasmic,membrane and nuclear staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Specific Publications For Anti-ADAM12 Antibody (A02264)
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Customer Q&As
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1 Customer Q&As for Anti-ADAM12 Antibody
Question
For this antibody A02264 what can be used as a positive control?
Verified Customer
Verified customer
Asked: 2014-04-01
Answer
We can suggest as positive control for the product A02264 antibody are fetal fibroblasts, Hela cells, A431 cells and human chondrosarcoma.
Boster Scientific Support
Answered: 2014-04-01
