Product Info Summary
SKU: | A02264 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ADAM12 Antibody
SKU/Catalog Number
A02264
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ADAM12 Antibody catalog # A02264. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAM12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02264)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Reactive Species
A02264 is reactive to ADAM12 in Human, Mouse, Rat
Applications
A02264 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
99.542kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of ADAM12 using Anti-ADAM12 Antibody (A02264).
Immunohistochemistry (IHC) analyzes of ADAM12 pAb in paraffin-embedded human breast carcinoma tissue at 1:50Click image to see more details
Figure 3. Immunohistochemistry validation of ADAM12 using Anti-ADAM12 Antibody (A02264).
Immunohistochemistry (IHC) analyzes of ADAM12 pAb in paraffin-embedded human stomach carcinoma tissue at 1:50Click image to see more details
Figure 1. Western blotting validation for Anti-ADAM12 Antibody A02264
Western blot (WB) analysis of ADAM12 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate
Lane2:Raw264.7 whole cell lysate
Lane3:H9C2 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ADAM12 (Source: Uniprot.org, NCBI)
Gene Name
ADAM12
Full Name
Disintegrin and metalloproteinase domain-containing protein 12
Weight
99.542kDa
Alternative Names
a disintegrin and metalloproteinase domain 12 (meltrin alpha); ADAM 12; ADAM metallopeptidase domain 12; ADAM12; disintegrin and metalloproteinase domain-containing protein 12; EC 3.4.24; EC 3.4.24.58; MCMP; MCMPMltna; Meltrin alpha; meltrin-alpha; MLTNA; MLTNEC 3.4.24.- ADAM12 ADAM12-OT1, CAR10, MCMP, MCMPMltna, MLTN, MLTNA ADAM metallopeptidase domain 12 disintegrin and metalloproteinase domain-containing protein 12|meltrin-alpha|metalloprotease-disintegrin 12 transmembrane
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADAM12, check out the ADAM12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADAM12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADAM12 Antibody (A02264)
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No publications found for A02264
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Customer Q&As
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1 Customer Q&As for Anti-ADAM12 Antibody
Question
For this antibody A02264 what can be used as a positive control?
Verified Customer
Verified customer
Asked: 2014-04-01
Answer
We can suggest as positive control for the product A02264 antibody are fetal fibroblasts, Hela cells, A431 cells and human chondrosarcoma.
Boster Scientific Support
Answered: 2014-04-01