Anti-ADAM28 Antibody Picoband™

Product Name

Anti-ADAM28 Antibody Picoband™

View all ADAM28 Antibodies

SKU/Catalog Number

A06873-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADAM28 Antibody Picoband™ catalog # A06873-1. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAM28 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06873-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ADAM28, different from the related mouse sequence by eleven amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06873-1 is reactive to ADAM28 in Human

Applications

A06873-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

87 kDa, 61 kDa

Calculated molecular weight

87.148kDa

Background of ADAM28

Disintegrin and metalloproteinase domain-containing protein 28 is an enzyme that in humans is encoded by the ADAM28 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. And this gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ADAM28 using anti-ADAM28 antibody (A06873-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: HELA whole cell lysates,
lane 2: K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM28 antigen affinity purified polyclonal antibody (Catalog # A06873-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM28 at approximately 87/61KD. The expected band size for ADAM28 is at 87KD.

Gene/Protein Information For ADAM28 (Source: Uniprot.org, NCBI)

Alternative Names

a disintegrin and metalloproteinase domain 28; ADAM metallopeptidase domain 28; ADAM23MDC-L; ADAM28; disintegrin and metalloproteinase domain-containing protein 28; Dtgn1; EC 3.4.24; EC 3.4.24.-; eMDC II; eMDCII; Epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich proteinII; MDCL; MDCLADAM 28; MDC-Lm; MDC-Ls; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L; metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L; Protein-L ADAM28 ADAM 28, MDC-L, MDCL, eMDC II, eMDCII ADAM metallopeptidase domain 28 disintegrin and metalloproteinase domain-containing protein 28|epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II|epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II|epididymis secretory sperm binding protein|metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L

For more info on ADAM28, check out the ADAM28 Infographic

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In this infographic, you will see the following information for ADAM28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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