Click image to see more details
Product Info Summary
| SKU: | A32410 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ADAP FYB1 Antibody
SKU/Catalog Number
A32410
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ADAP FYB1 Antibody (Catalog # A32410). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ADAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ADAP FYB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32410)
Host
Rabbit
Contents
ADAP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ADAP antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human ADAP. The immunogen is located within amino acids 640 - 690 of ADAP.
Reactive Species
A32410 is reactive to FYB in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
85.4 kDa
Background of FYB
The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-κB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32410 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 μg/mL.
Click image to see more details
Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 μg/mL.
Specific Publications For Anti-ADAP FYB1 Antibody (A32410)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ADAP FYB1 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-ADAP FYB1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
