Anti-ADCK5 Antibody

ADCK5 antibody

Boster Bio Anti-ADCK5 Antibody (Catalog # A16497). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A16497
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-ADCK5 Antibody

View all ADCK5 Antibodies

SKU/Catalog Number

A16497

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ADCK5 Antibody (Catalog # A16497). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADCK5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16497)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ADCK5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A16497 is reactive to ADCK5 in Human

Applications

A16497 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

65.828kDa

Background of ADCK5

The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).

Nusbaum C., Nature 439:331-335(2006).
The MGC Project Team, Genome Res. 14:2121-2127(2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For ADCK5 (Source: Uniprot.org, NCBI)

Gene Name

ADCK5

Full Name

Uncharacterized aarF domain-containing protein kinase 5

Weight

65.828kDa

Superfamily

protein kinase superfamily

Alternative Names

aarF domain containing kinase 5; EC 2.7.11.-; FLJ35454; MGC126708 ADCK5 aarF domain containing kinase 5 uncharacterized aarF domain-containing protein kinase 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADCK5, check out the ADCK5 Infographic

ADCK5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADCK5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-ADCK5 Antibody

Question

I have a question about product A16497, anti-ADCK5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16497 anti-ADCK5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-19

Question

Will A16497 anti-ADCK5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-02

Answer

As indicated on the product datasheet, A16497 anti-ADCK5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-02

Question

I see that the anti-ADCK5 antibody A16497 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-15

Question

Is a blocking peptide available for product anti-ADCK5 antibody (A16497)?

Verified Customer

Verified customer

Asked: 2018-06-21

Answer

We do provide the blocking peptide for product anti-ADCK5 antibody (A16497). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-06-21

Question

We are currently using anti-ADCK5 antibody A16497 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-14

Answer

The anti-ADCK5 antibody (A16497) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-14

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-ADCK5 antibody A16497. Let me know if you need anything else.

P. Walker

Verified customer

Asked: 2016-11-03

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-11-03

Order DetailsPrice
A16497-100uL

100uL

$360

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A16497
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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