Product Info Summary
| SKU: | M01101 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ADORA1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01101
BM5256 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ADORA1 Rabbit Monoclonal Antibody catalog # M01101. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADORA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01101)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACDG-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ADORA1 Receptor.
Reactive Species
M01101 is reactive to ADORA1 in Human
Observed Molecular Weight
37 kDa
Calculated molecular weight
36.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01101 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: A549 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ADORA1 expression in A549 cell lysate.
Specific Publications For Anti-ADORA1 Rabbit Monoclonal Antibody (M01101)
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Customer Q&As
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6 Customer Q&As for Anti-ADORA1 Rabbit Monoclonal Antibody
Question
We are currently using anti-ADORA1 Monoclonal antibody M01101 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The anti-ADORA1 Monoclonal antibody (M01101) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-17
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-ADORA1 Monoclonal antibody M01101. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-13
Question
Is this M01101 anti-ADORA1 Monoclonal antibody reactive to the isotypes of ADORA1?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The immunogen of M01101 anti-ADORA1 Monoclonal antibody is A synthesized peptide derived from human ADORA1 Receptor. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-11
Question
Does M01101 anti-ADORA1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
It shows on the product datasheet, M01101 anti-ADORA1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-19
Question
you antibody to test anti-ADORA1 Monoclonal antibody M01101 on human brain for research purposes, then I may be interested in using anti-ADORA1 Monoclonal antibody M01101 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Baker
Verified customer
Asked: 2017-07-24
Answer
The products we sell, including anti-ADORA1 Monoclonal antibody M01101, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-24
Question
Will anti-ADORA1 Monoclonal antibody M01101 work on dog WB with metanephric glomerulus?
A. Roberts
Verified customer
Asked: 2014-12-11
Answer
Our lab technicians have not validated anti-ADORA1 Monoclonal antibody M01101 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ADORA1 Monoclonal antibody M01101 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog metanephric glomerulus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-11


